GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.6 mg/mL
ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Rabbit anti Human Eg5 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function： Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Specifications
Product Name
Recombinant Eg5 Antibody - Anticorpo primario, specific to Eg5, Rabbit IgG
Sinonimi
EG5 | HKSP | KNSL1 | MCLMR | TRIP5 | EG5 antibody | HKSP antibody | KIF11 antibody | KIF11_HUMAN antibody | Kinesin family member 11 antibody | Kinesin like protein 1 antibody | Kinesin-like protein 1 antibody | Kinesin-like protein KIF11 antibody | Kines
Specifiche e purezza
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Specie ospite
Coniglio
Specificità
Eg5
Immunogeno
Recombinant protein of human Eg5 (AA 8-399)
Controllo positivo
WB: HeLa, A549, U2OS, K562 and A431 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Coniugazione
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Antigen affinity purified
Concentrazione
0.6 mg/mL
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
Recombinant Eg5 Antibody (Ab101062) - Western Blot All lanes: Recombinant Eg5 Antibody (Ab101062) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 119 kDa Observed band size: 121 kDa Exposure time: 5.9 s
Recombinant Eg5 Antibody (Ab101062) - IF/ICC Immunocytochemistry analysis of Eg5 (green) in HeLa using Recombinant Eg5 Antibody (Ab101062), and cells were counterstained with DAPI (blue).
Recombinant Eg5 Antibody (Ab101062) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Eg5 Antibody (Ab101062). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Applicazione
Applicazione
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Eg5 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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