GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.6 mg/mL
ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Eg5 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function： Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Specifications
Product Name
Recombinant Eg5 Antibody - Primary antibody, specific to Eg5, Rabbit IgG
Synonyms
EG5 | HKSP | KNSL1 | MCLMR | TRIP5 | EG5 antibody | HKSP antibody | KIF11 antibody | KIF11_HUMAN antibody | Kinesin family member 11 antibody | Kinesin like protein 1 antibody | Kinesin-like protein 1 antibody | Kinesin-like protein KIF11 antibody | Kines
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species
Rabbit
Specificity
Eg5
Immunogen
Recombinant protein of human Eg5 (AA 8-399)
Positive Control
WB: HeLa, A549, U2OS, K562 and A431 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.6 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Eg5 Antibody (Ab101062) - Western Blot All lanes: Recombinant Eg5 Antibody (Ab101062) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 119 kDa Observed band size: 121 kDa Exposure time: 5.9 s
Recombinant Eg5 Antibody (Ab101062) - IF/ICC Immunocytochemistry analysis of Eg5 (green) in HeLa using Recombinant Eg5 Antibody (Ab101062), and cells were counterstained with DAPI (blue).
Recombinant Eg5 Antibody (Ab101062) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Eg5 Antibody (Ab101062). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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