Recombinant LC3B Antibody - Anticorpo primario, specific to LC3B, Rabbit IgG

Cat. No.: Ab112866

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human LC3B Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant LC3B Antibody - Anticorpo primario, specific to LC3B, Rabbit IgG

Sinonimi

ATG8F antibody | Autophagy-related protein LC3 B antibody | Autophagy-related ubiquitin-like modifier LC3 B antibody | LC3B antibody | LC3II antibody | MAP1 light chain 3 like protein 2 antibody | MAP1 light chain 3-like protein 2 antibody | MAP1A/1BLC3 a

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular ener

Specie ospite

Coniglio

Specificità

LC3B

Immunogeno

A synthetic peptide derived from human LC3B (AA 1-110).

Controllo positivo

WB: HeLa, A549, MCF7, Raji, RAW 264.7 and C6 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

biological_products

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant LC3B Antibody (Ab112866)

Recombinant LC3B Antibody (Ab112866) - Western Blot
All lanes: Recombinant LC3B Antibody (Ab112866) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 42.3 s

Applicazione

Applicazione

Dilution info

WB

1/1000 - 1/2000

Obiettivi associati (umani)

MAP1LC3B Tbio Microtubule-associated proteins 1A/1B light chain 3B (0 Activities)

Discover Target: MAP1LC3B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant LC3B Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.