Recombinant LIS1 Antibody - Anticorpo primario, specific to PAFAH1B1, Rabbit IgG

Cat. No.: Ab113262

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.4 mg/mL

Application

WB

Host species

Coniglio

Reactive species

consiglio, Criceto, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human LIS1 Antibody, Recombinant, could be used for WB and so on.

Application:
WB: 1/500-1/1000

Protein Function
Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.

Specifications

Product Name

Recombinant LIS1 Antibody - Anticorpo primario, specific to PAFAH1B1, Rabbit IgG

Sinonimi

LIS 1 | LIS 2 | LIS-1 | LIS1 | LIS1_HUMAN | LIS2 | Lissencephaly 1 protein | Lissencephaly-1 protein | MDCR | MDS | PAF acetylhydrolase 45 kDa subunit | PAF AH 45 kDa subunit | PAF AH alpha | PAF-AH 45 kDa subunit | PAF-AH alpha | PAFAH alpha | PAFAH | PA

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.4 mg/mL

Specie ospite

Coniglio

Specificità

PAFAH1B1

Immunogeno

A synthetic peptide derived from human LIS1 (AA 64-77)

Controllo positivo

WB: HEK293, SK-OV-3 and SK-N-SH cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.4 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant LIS1 Antibody (Ab113262)

Recombinant LIS1 Antibody (Ab113262) - Western Blot
All lanes: Recombinant LIS1 Antibody (Ab113262) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 46 kDa
Exposure time: 195.1 s

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

Obiettivi associati (umani)

PAFAH1B1 Tbio Platelet-activating factor acetylhydrolase IB subunit alpha (0 Activities)

Discover Target: PAFAH1B1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant LIS1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.