Recombinant MCM2 Antibody - Anticorpo primario, specific to MCM2, Rabbit IgG

Cat. No.: Ab114532

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human MCM2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.

Application:

• WB: 1/500-1/1000
• IHC: 1/50-1/100 
• ICC/IF: 1/50-1/200          

Protein Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Post-translational

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

Specifiche

Product Name

Recombinant MCM2 Antibody - Anticorpo primario, specific to MCM2, Rabbit IgG

Sinonimi

Minichromosome maintenance protein 2 homolog|Nuclear protein BM28|BM28 antibody|CCNL 1 antibody|CCNL1 antibody|CDC like 1 antibody|cdc19 antibody|CDCL 1 antibody|CDCL1 antibody|Cell devision cycle like 1 antibody|Cyclin like 1 antibody|D3S3194 antibody|DNA replication licensing factor MCM2 antibody|KIAA0030 antibody|MCM 2 antibody|MCM2 antibody|MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody|MCM2 minichromosome maintenance deficient 2, mitotin antibody|MGC10606 antibody|Minichromosome maintenance complex component 2 antibody|Minichromosome maintenance deficient 2 (mitotin) antibody|Minichromosome maintenance protein 2 antibody|Minichromosome maintenance protein 2 homolog antibody|Mitotin antibody|Nuclear protein BM28 antibody|OTTHUMP00000216047 antibody|OTTHUMP00000216050 antibody|BM28|BM28MGC10606|CCNL 1|CCNL1|cdc19|CDCL1|CDCL1mitotin|KIAA0030MITOTIN|MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)|MCM2|minichromosome maintenance complex component 2|minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)|minichromosome maintenance deficient 2 (mitotin)|Mitotin|DFNA70

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Specie ospite

Coniglio

Specificità

MCM2

Immunogeno

A synthetic peptide derived from human MCM2 (AA 205-218)

Controllo positivo

WB: HeLa, A549, MCF7 and U2OS cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.2 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant MCM2 Antibody (Ab114532)

Recombinant MCM2 Antibody (Ab114532) - Western Blot
All lanes: Recombinant MCM2 Antibody (Ab114532) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 102 kDa
Observed band size: 120 kDa
Exposure time: 30.0 s

IF/ICC: Recombinant MCM2 Antibody (Ab114532)

Recombinant MCM2 Antibody (Ab114532) - IF/ICC
Immunocytochemistry analysis of MCM2 (green) in HeLa using Recombinant MCM2 Antibody (Ab114532), and cells were counterstained with DAPI (blue).

IHC: Recombinant MCM2 Antibody (Ab114532)

Recombinant MCM2 Antibody (Ab114532) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM2 Antibody (Ab114532). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Obiettivi associati (umani)

MCM2 Tbio DNA replication licensing factor MCM2 (0 Activities)

Discover Target: MCM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant MCM2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.