Recombinant NELF-B/COBRA1 Antibody - Anticorpo primario, specific to NELF-B, Rabbit IgG

Cat. No.: Ab117535

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human NELF-B Antibody, Recombinant, could be used for WB and so on.
Application
WB: 1/500-1/2000
Relevance
NELF-B is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be able to induce chromatin unfolding.

Specifications

Product Name

Recombinant NELF-B/COBRA1 Antibody - Anticorpo primario, specific to NELF-B, Rabbit IgG

Sinonimi

A730008L03Rik antibody | AB041607 antibody | AI663983 antibody | Cobra1 antibody | Cofactor of BRCA1 antibody | DKFZp586B0519 antibody | KIAA1182 antibody | Negative elongation factor B antibody | Negative elongation factor protein B antibody | Nelf b ant

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

NELF-B is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be abl

Specie ospite

Coniglio

Specificità

NELF-B

Immunogeno

A synthetic peptide derived from human NELF-B (AA 1-200).

Controllo positivo

WB: Jurkat, Hep G2 and MCF7 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant NELF-B Antibody (Ab117535)

Recombinant NELF-B Antibody (Ab117535) - Western Blot
All lanes: Recombinant NELF-B Antibody (Ab117535) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 100.0 s

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/2000;

Obiettivi associati (umani)

NELFB Tbio Negative elongation factor B (0 Activities)

Discover Target: NELFB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant NELF-B Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.