Recombinant PHAPI2/APRIL Antibody - Anticorpo primario, specific to ANP32B, Rabbit IgG

Cat. No.: Ab121553

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.3 mg/mL

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human PHAPI2/APRIL Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.

Specifications

Product Name

Recombinant PHAPI2/APRIL Antibody - Anticorpo primario, specific to ANP32B, Rabbit IgG

Sinonimi

Acidic (leucine rich) nuclear phosphoprotein 32 family member B | Acidic leucine rich nuclear phosphoprotein 32 family member B | Acidic leucine-rich nuclear phosphoprotein 32 family member B | Acidic nuclear phosphoprotein 32 family member B | Acidic pro

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Specie ospite

Coniglio

Specificità

ANP32B

Immunogeno

A synthetic peptide derived from human PHAPI2 (AA 146-159)

Controllo positivo

WB: K562, LNCaP, Jurkat, A549, U937, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: K562 cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.3 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant PHAPI2/APRIL Antibody (Ab121553)

Recombinant PHAPI2/APRIL Antibody (Ab121553) - Western Blot
All lanes: Recombinant PHAPI2/APRIL Antibody (Ab121553) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 28, 31 kDa
Exposure time: 17.3 s

IF/ICC: Recombinant PHAPI2/APRIL Antibody (Ab121553)

Recombinant PHAPI2/APRIL Antibody (Ab121553) - IF/ICC
Immunocytochemistry analysis of PHAPI2 (green) in K562 using Recombinant PHAPI2/APRIL Antibody (Ab121553) and DAPI (blue).

IHC: Recombinant PHAPI2/APRIL Antibody (Ab121553)

Recombinant PHAPI2/APRIL Antibody (Ab121553) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant PHAPI2/APRIL Antibody (Ab121553). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50 - 1/200;

Obiettivi associati (umani)

DCANP1 Tbio Dendritic cell nuclear protein 1 (0 Activities)

Discover Target: DCANP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ANP32B Tbio Acidic leucine-rich nuclear phosphoprotein 32 family member B (0 Activities)

Discover Target: ANP32B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant PHAPI2/APRIL Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.