Recombinant PP2A alpha/beta Antibody - Anticorpo primario, specific to PPP2CA/PPP2CB, Rabbit IgG

Cat. No.: Ab122709
Disponibile su ordine
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.2 mg/mL
Application
IF/ICC, IHC, WB
Host species
Coniglio
Reactive species
consiglio, Criceto, Mouse, Umano
Isotype
Rabbit IgG
Conjugation
Unconjugated
★
Size
Germania (EU)
USA*
Price
Qty
10μl
Ab122709-10μl
—
5 Disponibile
86,69€
50μl
Ab122709-50μl
—
2 Disponibile
225,53€
100μl
Ab122709-100μl
—
1 Disponibile
364,36€
1ml
Ab122709-1ml
Su ordinazione · 8–12 settimane
2.950,23€
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Panoramica

Rabbit anti Human PP2A alpha/beta Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Specifications

Product Name
Recombinant PP2A alpha/beta Antibody - Anticorpo primario, specific to PPP2CA/PPP2CB, Rabbit IgG
Sinonimi
PPP2CA | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | PP2A-alpha | Replication protein C | RP-C | PP2A A antibody | PP2A alpha antibody | PP2A B antibody | PP2A beta antibody | PP2A-alpha antibody | PP2A-beta antibody | PP2AA_
Specifiche e purezza
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Specie ospite
Coniglio
Specificità
PPP2CA/PPP2CB
Immunogeno
A synthetic peptide derived from human PP2A alpha/beta (AA 277-294).
Controllo positivo
WB: HeLa, C6, NIH/3T3, A431, Jurkat, HEK293, A549 and Raw264.7 cell lysates. IHC: Human lung cancer. ICC/IF: HeLa cells.
Coniugazione
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Immunogen affinity purified
Concentrazione
0.2 mg/mL
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini

Recombinant PP2A alpha/beta Antibody (Ab122709) - Western Blot
All lanes: Recombinant PP2A alpha/beta Antibody (Ab122709) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 34 kDa
Exposure time: 10.0 s

Recombinant PP2A alpha/beta Antibody (Ab122709) - IF
Immunocytochemistry analysis of PP2A alpha/beta (green) in HeLa cells using Recombinant PP2A alpha/beta Antibody (Ab122709) and cells were counterstained with DAPI (blue).

Recombinant PP2A alpha/beta Antibody (Ab122709) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PP2A alpha/beta Antibody (Ab122709). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione
ApplicazioneDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Obiettivi associati (umani)
PPP2CA Tchem Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PPP2CB Tbio Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PPP2CB Tbio Serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform (1 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Meccanismi d'azione
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDataOggetto
ZJ24F0911364Certificate of AnalysisJul 14, 2026 Ab122709
ZJ23F0901067Certificate of AnalysisSep 18, 2023 Ab122709
ZJ23F0901066Certificate of AnalysisSep 18, 2023 Ab122709
Domande frequenti e articoli
Calcolatori di soluzioni
Recensioni

Recensioni dei clienti

Domande frequenti

What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant PP2A alpha/beta Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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