Recombinant RAGE Antibody - Anticorpo primario, specific to RAGE, Rabbit IgG

Cat. No.: Ab124452

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human RAGE Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function：
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.

Specifications

Product Name

Recombinant RAGE Antibody - Anticorpo primario, specific to RAGE, Rabbit IgG

Sinonimi

Ager antibody | DAMA 358M23.4 antibody | MGC2235 antibody | MGC22357 antibody | RAGE_HUMAN antibody | Receptor for advanced glycation end products antibody | Receptor for advanced glycosylation end products antibody | advanced glycosylation end product-sp

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Specie ospite

Coniglio

Specificità

RAGE

Immunogeno

A synthetic peptide derived from human RAGE (AA 344-404).

Controllo positivo

WB: Mouse lung and Rat lung lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purifie

Concentrazione

See COA

Product Line

biological_products

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

WB: Mouse lung lysates.

Immagini

Western Blot: Recombinant RAGE Antibody (Ab124452)

Recombinant RAGE Antibody (Ab124452) - Western Blot
All lanes: Recombinant RAGE Antibody (Ab124452) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 25.9 s

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

Obiettivi associati (umani)

AGER Tchem Advanced glycosylation end product-specific receptor (0 Activities)

Discover Target: AGER

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant RAGE Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.