Recombinant RAP80 Antibody - Anticorpo primario, specific to RAP80, Rabbit IgG

Cat. No.: Ab124603

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human RAP80 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Specifiche

Product Name

Recombinant RAP80 Antibody - Anticorpo primario, specific to RAP80, Rabbit IgG

Sinonimi

Receptor-associated protein 80|Retinoid X receptor-interacting protein 110|Ubiquitin interaction motif-containing protein 1|BRCA1-A complex subunit RAP80 antibody|Nuclear zinc finger protein RAP80 antibody|OTTHUMP00000161441 antibody|OTTHUMP00000223372 antibody|OTTHUMP00000223374 antibody|RAP80 antibody|Receptor associated protein 80 antibody|Receptor-associated protein 80 antibody|Retinoid X receptor interacting protein 110 antibody|Retinoid x receptor interacting protein antibody|Retinoid X receptor-interacting protein 110 antibody|RIP110 antibody|Rxrip110 antibody|Ubiquitin interaction motif containing 1 antibody|Ubiquitin interaction motif containing protein 1 antibody|Ubiquitin interaction motif-containing protein 1 antibody|UIMC1 antibody|X2HRIP110 antibody|UIMC1|RAP80|X2HRIP110

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Specie ospite

Coniglio

Specificità

RAP80

Immunogeno

A synthetic peptide derived from human RAP80 (AA 26-40)

Controllo positivo

WB: MCF7 and U2OS cell lysates. IHC: Human breast cancer tissue. ICC/IF:HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.2 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant RAP80 Antibody (Ab124603)

Recombinant RAP80 Antibody (Ab124603) - Western Blot
All lanes: Recombinant RAP80 Antibody (Ab124603) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 15.9 s

IF/ICC: Recombinant RAP80 Antibody (Ab124603)

Recombinant RAP80 Antibody (Ab124603) - IF/ICC
Immunocytochemistry analysis of RAP80 (green) in HeLa using Recombinant RAP80 Antibody (Ab124603), and cells were counterstained with DAPI (blue).

IHC: Recombinant RAP80 Antibody (Ab124603)

Recombinant RAP80 Antibody (Ab124603) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant RAP80 Antibody (Ab124603). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Obiettivi associati (umani)

UIMC1 Tbio BRCA1-A complex subunit RAP80 (0 Activities)

Discover Target: UIMC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant RAP80 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.