GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.8 mg/mL
ExactAb™, Validated, Recombinant, 0.8 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human RING2/RING1B/RNF2 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in × chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random × inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
Specifications
Product Name
Recombinant RING2/RING1B/RNF2 Antibody - Primary antibody, specific to RING2/RING1B/RNF2, Rabbit IgG
Synonyms
EC 2.3.2.27 | HIP2-interacting protein 3 | Huntingtin-interacting protein 2-interacting protein 3 | Protein DinG | RING finger protein 1B | RING finger protein 2 | RING finger protein BAP-1 | RING1b | RING-type E3 ubiquitin transferase RING2 | BAP 1 antib
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.8 mg/mL
Host species
Rabbit
Specificity
RING2/RING1B/RNF2
Immunogen
A synthetic peptide derived from human RING2 / RING1B / RNF2 (AA 118-131)
Positive Control
WB: HeLa, A549, U-2 OS, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.8 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 38 kDa Observed band size: 41 kDa Exposure time: 137.7 s
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 38 kDa Observed band size: 41 kDa Exposure time: 60.0 s
Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RING2/RING1B/RNF2 Antibody (Ab125386). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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