Recombinant STAT4 Antibody - Anticorpo primario, specific to STAT4, Rabbit IgG

Cat. No.: Ab129436

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human STAT4 Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant STAT4 Antibody - Anticorpo primario, specific to STAT4, Rabbit IgG

Sinonimi

DPMC | SLEB11 | signal transducer and activator of transcription 4 | STAT4 | Signal transducer and activator of transcription 4 antibody | SLEB11 antibody | STAT4 antibody | STAT4_HUMAN antibody

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

Specie ospite

Coniglio

Specificità

STAT4

Immunogeno

A synthetic peptide derived from human STAT4 (AA 700-748)

Controllo positivo

WB: HeLa, Jurkat, Hep G2, MCF7 and MOLT-4 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant STAT4 Antibody (Ab129436)

Recombinant STAT4 Antibody (Ab129436) - Western Blot
All lanes: Recombinant STAT4 Antibody (Ab129436) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 48, 86 kDa
STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors (PMID:22383755). The DNA-binding domain can bind to DNA which affects its migration in Western blot experiments.
Exposure time: 86.5 s 

IF/ICC: Recombinant STAT4 Antibody (Ab129436)

Recombinant STAT4 Antibody (Ab129436) - IF/ICC
IF analysis of Recombinant STAT4 Antibody (Ab129436) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant STAT4 Antibody (Ab129436) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/200 ;

Obiettivi associati (umani)

STAT4 Tchem Signal transducer and activator of transcription 4 (0 Activities)

Discover Target: STAT4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant STAT4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.