Recombinant STING Antibody - Anticorpo primario, specific to STING, Rabbit IgG

Cat. No.: Ab129563

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.4 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human STING Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500 – 1/2000

IHC: 1/50-/100

IF/ICC: 1/200

Protein Function

Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

Specifications

Product Name

Recombinant STING Antibody - Anticorpo primario, specific to STING, Rabbit IgG

Sinonimi

endoplasmic reticulum IFN stimulator antibody | Endoplasmic reticulum interferon stimulator antibody | ERIS antibody | FLJ38577 antibody | hMITA antibody | hSTING antibody | Mediator of IRF3 activation antibody | MITA antibody | Mitochondrial mediator of

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.4 mg/mL

Specie ospite

Coniglio

Specificità

STING

Immunogeno

Recombinant protein of human STING (AA 273-377).

Controllo positivo

WB: K562, THP-1, HT-29 and Jurkat cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.4 mg/mL

Product Line

biological_products

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant STING Antibody (Ab129563)

Recombinant STING Antibody (Ab129563) - Western Blot
All lanes: Recombinant STING Antibody (Ab129563) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 42 kDa
Observed band size: 38 kDa
Exposure time: 1.9 s

IHC: Recombinant STING Antibody (Ab129563)

Recombinant STING Antibody (Ab129563) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant STING Antibody (Ab129563). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant STING Antibody (Ab129563)

Recombinant STING Antibody (Ab129563) - IF/ICC

IF analysis of Recombinant STING Antibody (Ab129563) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant STING Antibody (Ab129563) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI(D1372407)  (blue).

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

IHC

1/50 - 1/100

IF/ICC

1/200

Obiettivi associati (umani)

TMEM173 Tchem Stimulator of interferon genes protein (0 Activities)

Discover Target: TMEM173

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant STING Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.