Recombinant TCF7L2 Antibody - Anticorpo primario, specific to TCF7L2, Rabbit IgG

Cat. No.: Ab130508

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human TCF7L2 Antibody, Recombinant, could be used for WB, IHC and so on.

Specifications

Product Name

Recombinant TCF7L2 Antibody - Anticorpo primario, specific to TCF7L2, Rabbit IgG

Sinonimi

E2 2; HMG box transcription factor 4; hTCF 4; Immunoglobulin transcription factor 2; ITF2; SEF2 1; SEF2 1A; SEF2 1B; SEF2; T cell factor 4; T cell specific HMG box; T cell specific transcription factor 4; TCF 4; TCF4; TCF7L2; TCF7L2 protein; hTCF-4; T-cel

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with seve

Specie ospite

Coniglio

Specificità

TCF7L2

Immunogeno

A synthetic peptide derived from human TCF7L2 (AA 1-50)

Controllo positivo

WB: Jurkat, K562, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human colonic cancer tissue. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant TCF7L2 Antibody (Ab130508)

Recombinant TCF7L2 Antibody (Ab130508) - Western Blot
All lanes: Recombinant TCF7L2 Antibody (Ab130508) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 62 kDa
Exposure time: 6.5 s

IHC: Recombinant TCF7L2 Antibody (Ab130508)

Recombinant TCF7L2 Antibody (Ab130508) - IHC
Immunohistochemical analysis of paraffin-embedded human colonic cancer, using Recombinant TCF7L2 Antibody (Ab130508).

IF/ICC: Recombinant TCF7L2 Antibody (Ab130508)

Recombinant TCF7L2 Antibody (Ab130508) - IF/ICC
IF analysis of Recombinant TCF7L2 Antibody (Ab130508) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant TCF7L2 Antibody (Ab130508) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Applicazione

Applicazione

Dilution info

IHC

1/100 - 1/200

WB

1/1000 - 1/2000

IF/ICC

1/200

Obiettivi associati (umani)

TCF7L2 Tbio Transcription factor 7-like 2 (0 Activities)

Discover Target: TCF7L2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant TCF7L2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.