Recombinant U1-C Antibody - Anticorpo primario, specific to SNRPC, Rabbit IgG

Cat. No.: Ab133164

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, 0.6 mg/mL

Application

WB

Host species

Coniglio

Reactive species

consiglio, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human U1-C Antibody, Recombinant, could be used for WB and so on.

Application:

WB: 1/500 - 1/1000

Protein Function

This protein is associated with snRNP U1.

Specifiche

Product Name

Recombinant U1-C Antibody - Anticorpo primario, specific to SNRPC, Rabbit IgG

Sinonimi

FLJ20302 antibody | OTTHUMP00000016238 antibody | OTTMUSP00000026897 antibody | RU1C_HUMAN antibody | Small nuclear ribonucleoprotein polypeptide C antibody | Snrpc antibody | U1 C antibody | U1 small nuclear ribonucleoprotein 1C antibody | U1 small nucle

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.6 mg/mL

Specie ospite

Coniglio

Specificità

SNRPC

Immunogeno

A synthetic peptide derived from human U1-C (AA 146-159)

Controllo positivo

WB: THP-1, Jurkat, HeLa, K562, HEK293, RAW 264.7 and C6 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.6 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant U1-C Antibody (Ab133164)

Recombinant U1-C Antibody (Ab133164) - Western Blot
All lanes: Recombinant U1-C Antibody (Ab133164) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 21 kDa
Exposure time: 80.0 s

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

Obiettivi associati (umani)

SNRPC Tbio U1 small nuclear ribonucleoprotein C (0 Activities)

Discover Target: SNRPC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ZC3H15 Tbio Zinc finger CCCH domain-containing protein 15 (0 Activities)

Discover Target: ZC3H15

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Tested/validated applications, recommended dilutions, positive controls, and protocol specifics are not specified for this item; refer to the CoA/Spec Sheet.

General guidance (literature/common practice; not item-specific):

• WB: Start with 1:500–1:2,000 primary dilutions; optimize based on signal-to-noise. Include nuclear extract from actively transcribing cells as a positive control for U1 snRNP proteins.
• IP: 1–5 µg antibody per mg lysate protein as a starting point; maintain cold conditions and include RNase inhibitors to preserve snRNP integrity.
• IF/ICC: 1:200–1:1,000 primary dilutions; optimize fixation/permeabilization.

These suggestions are generic starting points and do not constitute validated instructions for SKU Ab133164.

Biological Roles

Target overview (literature/general): U1-C, encoded by SNRPC, is an essential protein component of the U1 snRNP particle within the spliceosome.

• Function in pre-mRNA splicing:
  • Stabilizes the interaction of U1 snRNP with the 5′ splice site during early spliceosome assembly (E complex), working with U1-70K (SNRNP70) and the Sm core proteins.
  • Contributes to 5′ splice site recognition fidelity; U1-C contacts the splice site through protein–RNA and protein–protein interfaces.
• Cellular localization: Predominantly nuclear, enriched in speckles/CBs where snRNP biogenesis and splicing factors concentrate.
• Complex membership:
  • U1 snRNP contains U1 snRNA, U1-70K, U1-A, U1-C, and a heptameric Sm ring; U1-C is integral for proper assembly and functional conformation.
• Biological context:
  • Spliceosome dynamics influence alternative splicing and transcriptome diversity; perturbation of U1 snRNP components can affect gene expression programs.
• Experimental implications:
  • Antibodies against U1-C enable probing of early spliceosome formation, co-IP of U1-associated factors, and visualization of nuclear RNP architecture.

Note: The above describes the biology of the target protein in general. It does not constitute item-specific validation or performance claims for SKU Ab133164.

Buffer Applications

Buffers are central to antibody performance and stability. The following are general, practical guidelines for antibody-based assays.

• Storage buffers (general recommendations):
  • PBS, pH 7.2–7.6, 150 mM NaCl; optional 30–50% glycerol for -20°C storage to avoid freezing and reduce aggregation.
  • Include 0.02–0.05% preservative only if required by your biosafety protocols; preserve-free formats reduce hazards but require sterile technique.
• Working/dilution buffers:
  • PBS or TBS with 0.01–0.05% Tween-20 to minimize nonspecific interactions in WB/ELISA.
  • Add 1–5% BSA for blocking/dilution in WB and ELISA; avoid carrier proteins when they may interfere with downstream MS or conjugation.
• IP/Co-IP buffers:
  • Non-denaturing lysis buffers such as 20–50 mM Tris, 150 mM NaCl, 0.5–1% NP-40 or Triton X-100, protease and RNase inhibitors for RNP complexes (e.g., U1 snRNP).
• IF/ICC buffers:
  • Fixation: 3–4% paraformaldehyde in PBS; permeabilization 0.1–0.5% Triton X-100 or saponin depending on epitope accessibility; block with 3% BSA or serum.

Item-specific buffer composition and any additives present in the vial are not specified for this product; consult the CoA/Spec Sheet.

Green Alternatives

Greener practices for antibody workflows focus on minimizing hazardous preservatives, reducing cold-chain intensity, and waste management. These are general considerations and not item-specific requirements.

• Preservation and additives:
  • Prefer low-toxicity preservatives or preservative-free formats when biosafety allows; avoid unnecessary azide streams into aqueous waste. If azide is present, collect separately and dispose properly.
  • Use glycerol and sugar-based stabilizers instead of organic solvents for freeze protection.
• Process efficiency:
  • Plan experiments to minimize freeze–thaw cycles and aliquot to reduce sample loss and re-order frequency.
  • Employ low-protein-binding plastics to reduce waste due to adsorption and improve yield.
• Energy use:
  • Consolidate freezer space and use high-efficiency -20°C/-80°C units; defrost/maintain gaskets to lower power draw.
• Packaging and consumables:
  • Choose reusable gel boxes, stain trays, and secondary containers when compatible with biosafety requirements.

Comparison (general):

• Conventional practice: Broad use of sodium azide and single-use plastics; frequent dry-ice shipments.
• Greener practice: Preservative-conscious formulations, strategic aliquoting, reduced-plastic workflows, and shipment scheduling to minimize repeat cold-chain legs.

Item-specific formulation, preservatives, and shipping options beyond those stated are not specified; refer to CoA/Spec Sheet.

Pharmaceutical Uses

This product is designated For research use only and is not intended for human or veterinary use, diagnostic procedures, or as a therapeutic.

• Formulation/excipient status: Not applicable; no pharmacopeial status is claimed or implied for this research reagent.
• Manufacturing/CMC context: Not applicable. No claims are made regarding GMP production, sterility suitable for clinical administration, or endotoxin limits compliant with parenteral use.

For any translational work or assay development destined for regulated environments, researchers should source appropriate GMP-grade materials and follow relevant pharmacopeial and regulatory guidelines.

Physical Properties

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. Typical IgG is ~150 kDa (literature/general).
• Isoelectric point (pI): Not specified. Many IgGs exhibit pI ~6–9 depending on sequence/glycosylation (literature/general).
• Solubility: Antibodies are readily soluble in aqueous buffered saline (PBS/TBS) at neutral pH; avoid low ionic strength and extreme pH (literature/general).
• Extinction coefficient: Not specified for this item; refer to CoA/Spec Sheet. Typical A280 quantification uses ε derived from sequence (literature/general).
• Stability considerations (literature/general):
  • Sensitive to repeated freeze–thaw; aggregation risk increases upon temperature cycling and at elevated temperatures.
  • Optimal stability near neutral pH, moderate ionic strength; stabilizers may include glycerol, sugars, or carrier proteins. Presence/type not specified for this item.
• Storage (from Product Data): Store at -20°C. Avoid repeated freezing and thawing. Shipped in an ice chest with ice pads.

Note: No item-specific refractive index, density, or small-molecule constants apply to antibody biologics. For exact concentration, buffer composition, and any additives (e.g., glycerol, carrier protein, preservatives), consult the CoA/Spec Sheet.

Quality and Grades

• Item-specific grade (from Product Data): Recombinant, ExactAb™, Validated.
• What this generally means:
  • Recombinant: Sequence-defined production in a controlled expression system to improve lot-to-lot consistency versus hybridoma or polyclonal sources. Enables defined isotype and sequence traceability.
  • ExactAb™: Branding indicative of engineered/recombinant antibody production and QC framework. Specific platform details and sequence disclosures are not provided here; refer to CoA/Spec Sheet for item-level attributes.
  • Validated: Indicates internal verification against the intended antigen under one or more assay formats. Exact tested applications and species reactivity are not specified here; see the CoA/Spec Sheet for details.
• QC considerations typically associated with recombinant antibodies (general):
  • Identity confirmation (mass/peptide mapping), purity by SDS-PAGE/SEC, concentration by A280, and binding performance in reference assays.
  • Bioburden/endotoxin control for research use; pharmacopeial compliance is not implied.
• What is not specified for this item (consult CoA):
  • Host expression system, isotype/subclass, clonality, exact buffer composition, preservative/stabilizer content, concentration, and validated application list.

Summary: This is a recombinant, ExactAb™-branded, validated research antibody targeting U1-C, supplied for research use only, with quality attributes focused on reproducibility and performance verification.

Reaction and Applications

As a recombinant research antibody, this product is used in immunoassay and affinity workflows rather than chemical reactions.

• Typical application families (literature/general; not item-specific performance claims):
  • Immunoblotting (Western blot) to detect U1-C in nuclear extracts or whole cell lysates.
  • Immunoprecipitation (IP) or co-IP of the U1 snRNP complex to study spliceosome assembly and RNA-protein interactions.
  • Immunofluorescence (IF)/immunocytochemistry (ICC) to visualize nuclear speckles and snRNP localization.
  • ELISA or capture assays for quantitative binding when antigen is immobilized/recombinant.
• Tips for successful use (general):
  • Validate antibody concentration empirically across a dilution series; include positive controls such as cell lines with high SNRPC expression.
  • For IP of ribonucleoproteins, include RNase inhibitors and maintain low temperatures; use gentle, non-denaturing lysis buffers (e.g., NP-40 at 0.1–1%).
  • For IF, optimize fixation (paraformaldehyde vs methanol) and permeabilization (Triton X-100 vs saponin) to preserve nuclear structures.
• Controls and specificity assessment (general):
  • Include peptide/antigen competition where feasible.
  • Use knockdown/knockout lines (siRNA/CRISPR) as negative controls for specificity.

Note: The Manufacturer Applications field for this item is not populated; consult the CoA/Spec Sheet for any item-specific, validated applications.

Reaction Conditions

Not applicable in the conventional synthetic chemistry sense. Antibody use involves assay conditions rather than chemical reactions. For general assay condition guidance:

• Temperature: Keep on ice during setup; incubations commonly at room temperature or 4°C depending on assay (e.g., overnight 4°C for IP or primary antibody incubations in WB/IF) — literature/general.
• Buffers: Neutral pH, isotonic saline buffers (PBS/TBS), optional mild detergents for blotting and IP — literature/general.
• Timing: Incubation times vary by assay and should be empirically optimized.

Item-specific, validated conditions are not provided; refer to the CoA/Spec Sheet and SDS.

Safety and Handling

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS for authoritative safety information.
• General laboratory PPE (good practice):
  • Wear lab coat, disposable nitrile gloves, and safety glasses.
  • Work on clean surfaces using low-protein-binding plastics to minimize adsorption losses.
• Handling precautions (general for antibodies):
  • Avoid repeated freeze–thaw cycles to prevent aggregation and activity loss.
  • Mix gently; do not vortex harshly. Keep on ice during use to limit proteolysis.
  • Use sterile technique to avoid microbial contamination of the vial.
• Incompatibilities (general):
  • Extreme pH (<5 or >9) and high shear can denature antibodies.
  • Metal-catalyzed oxidation and light exposure can degrade proteins; store in the dark when possible.
• Preservatives note:
  • Many antibody preparations may contain low levels of preservatives such as sodium azide or isothiazolinones to limit microbial growth; presence/amounts are not specified for this item. If present, handle and dispose according to institutional and local regulations.
• First-aid overview (general):
  • Skin/eye contact: Rinse with water. Remove contaminated clothing.
  • Ingestion/inhalation: Seek medical attention as needed.

Always consult the product SDS and institutional biosafety guidelines before use.

Solvent Selection

Solvent concepts for antibodies refer to aqueous buffers rather than organic solvents.

• Primary diluents (literature/general):
  • PBS (10–50 mM phosphate, 150 mM NaCl, pH ~7.2–7.6): Widely used for storage and assay dilution; isotonic and gentle on IgG structure.
  • TBS (Tris-buffered saline, pH ~7.4–7.6): Alternative to PBS; preferred when phosphate interferes (e.g., certain phospho-assays or calcium-dependent systems).
• Additives to improve stability (general):
  • 0.01–0.05% Tween-20 for assay dilutions to reduce nonspecific binding; avoid in protein A/G interactions where detergents may interfere.
  • Protein carriers (1–5% BSA or 1–3% nonfat dry milk) for blotting; avoid carriers in applications where they interfere (e.g., mass spectrometry, certain conjugations).
  • Cryoprotectant: 30–50% glycerol for -20°C storage to prevent freezing and reduce aggregation.
• When to choose which buffer (general guidance):
  • PBS for most immunoassays and storage; TBS for alkaline phosphatase-based assays or when phosphate is problematic.
  • HEPES-buffered saline (pH-stable near 7.4) for room-temperature workflows with CO2 exposure.
• Compatibility notes:
  • Avoid strong organic solvents, chaotropes, and extremes of pH unless for controlled elution or regeneration in affinity workflows.

Item-specific buffer composition and formulation: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Item-specific storage (from Product Data):
  • Store at -20°C.
  • Avoid repeated freezing and thawing.
  • Shipped in an ice chest with ice pads.
• Handling recommendations (general):
  • Upon receipt, briefly centrifuge to collect contents, inspect for integrity, and aliquot into low-protein-binding tubes to avoid freeze–thaw cycles.
  • Keep on ice during use; protect from light if conjugated (conjugation status not specified for this item).
• Reconstitution:
  • Reconstitution details (e.g., volume, buffer) are not specified for this item; refer to CoA/Spec Sheet.
  • General guidance if provided as lyophilized powder (literature/common practice): Reconstitute with sterile PBS or water to the desired concentration, gently invert to dissolve, then adjust to storage buffer (e.g., PBS with 30–50% glycerol for -20°C) as needed.
• Short- and long-term storage (general):
  • Short term (≤1 week): 4°C in sterile buffer may be acceptable.
  • Long term: -20°C (or -80°C for maximum stability) in aliquots; adding cryoprotectant reduces freeze–thaw stress.

Buffer composition, concentration, preservative content, and appearance are not specified; consult the CoA/Spec Sheet for item-specific instructions.

Structure and Identity

Recombinant U1-C Antibody is a sequence-defined immunoglobulin reagent targeting the U1-C (SNRPC) protein of the U1 small nuclear ribonucleoprotein (snRNP) complex.

• Item identifiers
  • SKU: Ab133164
  • Product name: Recombinant U1-C Antibody
  • CAS: Not assigned for antibodies
  • CID / InChIKey / SMILES: Not applicable to protein biologics
• Molecule type (literature/general):
  • Typical antibody framework: IgG-class immunoglobulin composed of 2 heavy chains and 2 light chains, with inter- and intrachain disulfides forming a Y-shaped homodimeric glycoprotein.
  • Domain architecture: variable domains (VH/VL) for antigen binding and constant domains (CH1–3, CL) for stability and Fc functions; flexible hinge between Fab and Fc.
• Structural features (literature/general):
  • Glycosylation: Fc N-glycans common for mammalian-expressed recombinant antibodies (pattern host-dependent). Specific glycoform not specified for this item; refer to CoA/Spec Sheet.
  • Antigen recognition: paratope formed by CDR loops within Fab domains; binds epitopes within U1-C protein.
• 2D description in words (literature/general):
  • A symmetric Y-shaped macromolecule; each arm (Fab) presents a variable region forming an antigen-binding site; the stem (Fc) mediates effector/handling properties. Overall anionic-to-neutral surface at neutral pH depending on sequence and glycosylation.

Item-specific molecular formula and exact molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not typically applicable. Antibodies are biological affinity reagents rather than synthetic building blocks or reagents for chemical transformations. For this item, see the sections on Reaction & Applications and Buffer Applications for practical laboratory use in immunoassays and affinity workflows.

Target Specificity

• Antigen/target: Not specified for this item; refer to CoA/Spec Sheet. Product name indicates U1-C (SNRPC) as the intended target.
• Clone, isotype/subclass, species reactivity, and epitope: Not specified for this item; refer to CoA/Spec Sheet.

No additional target specificity details are provided in the Product Data.

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant U1-C Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.