Recombinant U2AF65 Antibody - Anticorpo primario, specific to U2AF2, Rabbit IgG

Cat. No.: Ab133178

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human U2AF65 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.
Post-translational
Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.

Specifiche

Product Name

Recombinant U2AF65 Antibody - Anticorpo primario, specific to U2AF2, Rabbit IgG

Sinonimi

hU2AF(65) antibody | hU2AF65 antibody | Splicing factor U2AF 65 kDa subunit antibody | U2 (RNU2) small nuclear RNA auxiliary factor 2 antibody | U2 auxiliary factor 65 kDa subunit antibody | U2 small nuclear ribonucleoprotein auxiliary factor (65kD) antib

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Specie ospite

Coniglio

Specificità

U2AF2

Immunogeno

A synthesized peptide derived from human U2AF65 (AA 415-475)

Controllo positivo

WB: HeLa, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant U2AF65 Antibody (Ab133178)

Recombinant U2AF65 Antibody (Ab133178) - Western Blot
All lanes: Recombinant U2AF65 Antibody (Ab133178) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 40, 59 kDa
Exposure time: 19.7 s

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

Obiettivi associati (umani)

U2AF2 Tbio Splicing factor U2AF 65 kDa subunit (0 Activities)

Discover Target: U2AF2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Tested applications, recommended dilutions, and positive controls are not provided in the Product Data for Ab133178.

• Manufacturer Applications (verbatim): Not specified.
• Please consult the CoA/Spec Sheet for:
  • Validated applications (e.g., WB, IF, IHC, IP, RIP, ELISA), if available for this item.
  • Recommended dilutions, incubation times, and blocking conditions.
  • Positive control lysates or tissues.
  • Species cross-reactivity and epitope information.

General note: Because this is a recombinant ExactAb™ product, standardized performance documentation is typically provided; obtain the latest lot-specific CoA for authoritative protocols.

Biological Roles

Target: U2AF65 (U2AF2) — general biology overview (literature/general; not product-specific performance claims).

• Function: U2AF65 is the 65-kDa subunit of the U2 small nuclear RNA auxiliary factor (U2AF). It binds the polypyrimidine tract upstream of the 3′ splice site in pre-mRNA, recruiting the U2 snRNP to form the prespliceosome.
• Complex formation: Cooperates with U2AF35 (U2AF1), which recognizes the AG dinucleotide at the 3′ splice site. Together they stabilize early spliceosome assembly (E complex to A complex transitions).
• Domains: Contains RNA recognition motifs (RRMs) and low-complexity regions that mediate RNA binding and protein–protein interactions; exhibits regulated phosphorylation influencing splicing decisions.
• Cellular localization: Predominantly nuclear, enriched in speckle-like compartments associated with splicing (immunocytochemistry literature).
• Biological significance: Implicated in alternative splicing regulation across diverse transcripts; changes in U2AF65 levels or activity can alter exon inclusion/exclusion and impact gene expression programs.
• Research relevance: A key marker for studies on spliceosome dynamics, RNA processing, and the impact of signaling pathways on co-transcriptional splicing.

Note: The species origin of the immunogen and cross-reactivity profile for Ab133178 are not specified; consult the CoA/Spec Sheet for species reactivity and epitope details.

Buffer Applications

Buffer systems relevant to antibody handling (general guidance; not product-specific):

• Storage buffers:

  • PBS, pH 7.2–7.4: Common for maintaining antibody stability and isotonicity. Optional stabilizers (glycerol 10–50%, sugars, BSA) can enhance freeze–thaw tolerance. For Ab133178, actual formulation is not specified.
  • TBS, pH 7.4–8.0: Often used when phosphate can interfere with certain detection chemistries.

• Assay buffers:

  • WB: TBS-T or PBS-T (0.05–0.1% Tween-20) for washes; blocking with 3–5% BSA or nonfat milk depending on detection system.
  • IF/IHC: PBS/TBS with carrier protein and mild detergent for permeabilization (e.g., 0.1–0.3% Triton X-100) as needed; fixation and antigen retrieval conditions depend on sample.
  • IP/RIP: Non-denaturing buffers with salt (150–300 mM NaCl) and protease/RNase inhibitors where relevant; Mg2+ and RNase inhibitors are standard for RNA-binding protein studies.

• Compatibility cautions:

  • Avoid sodium azide in HRP-based detection (enzyme inhibition) and in live-cell assays.
  • Avoid primary amines (e.g., Tris) during NHS-ester conjugation steps.

For Ab133178, use buffer systems recommended in the CoA/Spec Sheet once provided; details are not specified in the Product Data.

Green Alternatives

Antibody reagents generally pose low environmental hazard compared to organic solvents, but buffer components and preservatives can impact safety and downstream disposal.

General greener choices (literature/general):

• Preservatives:

  • Sodium azide: Effective antimicrobial but acutely toxic and hazardous to plumbing metals; consider azide-free formulations when feasible.
  • ProClin/bronopol: Lower acute toxicity than azide but still hazardous; handle with care.
  • Preservative-free: Greener at point-of-use; requires sterile technique and aliquoting to prevent contamination.

• Buffer systems:

  • Use phosphate or HEPES buffers prepared with ultrapure water; avoid volatile organic cosolvents.
  • Consider glycerol-free storage when ultra-cold storage (≤−70°C) is available to reduce carbon footprint from viscous cryoprotectants.

Trade-offs (general):

• Azide-free or preservative-free formats enhance compatibility with live-cell assays and enzyme conjugations but demand stricter aseptic handling and shorter bench stability.
• Glycerol improves freeze–thaw resilience but increases viscosity and can complicate lyophilization.

For Ab133178, the presence or absence of preservatives, carrier proteins, or glycerol is not specified; refer to the CoA/Spec Sheet to align environmental and experimental requirements.

Pharmaceutical Uses

This product is supplied strictly for research use only (per Product Data) and is not intended for diagnostic or therapeutic applications.

General, non-clinical context (literature/general):

• Antibodies like this can serve as process analytics reagents in biopharmaceutical R&D, for example as controls in Western blots, ELISAs, or immunocapture-MS workflows studying RNA-binding proteins.
• As characterization tools, anti-U2AF65 reagents may be used to benchmark assay performance or to develop protocol steps for target engagement studies in early discovery.

No pharmacopeial status, excipient role, or clinical manufacturing use is specified for Ab133178. Do not use in humans or for diagnostic procedures. Consult institutional guidelines for using research-only antibodies in GLP or GMP-adjacent environments.

Physical Properties

Item-specific physicochemical parameters are not provided for this antibody preparation.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight (antibody): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general values for typical IgG-class antibodies (for context only; not product specifications):

• Typical MW: ~150 kDa for full-length IgG; Fab ~50 kDa; scFv ~25–30 kDa (literature).
• Solubility: Freely soluble in aqueous buffers (e.g., PBS, TBS) at neutral pH; stability influenced by salt, pH, and excipients (general).
• Isoelectric point (pI): Commonly in the range pH 6–9 depending on sequence/glycosylation (literature).
• Storage buffer components often include salts (PBS or TBS), pH 7–8, optional stabilizers such as glycerol, BSA, sugars, and preservatives (e.g., sodium azide) — specific formulation for this item is not specified.

Note: Antibodies are biological macromolecules; standard solvent-like properties (bp, mp, density, refractive index, logP) are not applicable. For exact composition, concentration, and excipients of Ab133178, consult the CoA/Spec Sheet.

Quality and Grades

• Item-specific designations (per Product Data):

  • Grade/Purity: Recombinant, ExactAb™, Validated.

• What this means (general definitions; not product specifications):

  • Recombinant: Produced from a defined DNA sequence in a controlled expression system, enabling consistency across lots and eliminating animal-to-animal variability typical of hybridoma-derived or polyclonal reagents.
  • ExactAb™: Proprietary naming commonly used to denote a platform of sequence-defined recombinant antibodies with standardized production and quality controls; consult the manufacturer’s documentation for criteria specific to this line.
  • Validated: Indicates the antibody has undergone internal analytical verification for performance; however, the specific validated applications (WB, IF, IHC, IP, etc.), species reactivity, and performance metrics for this item are not specified; refer to CoA/Spec Sheet.

• Implications for users:

  • Lot-to-lot reproducibility and reduced background are typical benefits of recombinant formats (literature/general).
  • If the product is preservative-free or carrier-free, that would affect downstream conjugations or cell-based assays; for Ab133178 such formulation details are not specified.
  • For regulated workflows, retain CoA/SDS and document lot numbers; this item is for research use only (per Product Data).

Reaction and Applications

This product is a biological affinity reagent and is not used as a stoichiometric or catalytic reagent in chemical synthesis.

General research applications for anti-U2AF65 antibodies (literature/general; not product-specific claims):

• Western blotting (WB) to detect endogenous U2AF65 levels and assess spliceosome component abundance.
• Immunoprecipitation (IP) or RNA immunoprecipitation (RIP) to capture U2AF65-containing ribonucleoprotein complexes and map RNA interactions.
• Immunofluorescence (IF/IHC) to visualize nuclear localization patterns of U2AF65 in fixed cells or tissues.
• Chromatin or crosslinking-based assays (CLIP iCLIP/eCLIP variants) for profiling RNA-binding sites when paired with crosslinking workflows.

Note: The specific validated applications for Ab133178 are not provided in the Product Data. Users should consult the CoA/Spec Sheet for tested applications, optimal dilutions, and species reactivity for this exact item.

Reaction Conditions

Not applicable for chemical reaction conditions. This product is an affinity reagent used in bioanalytical assays rather than synthetic chemistry.

General assay condition notes (literature/general; not product-specific):

• WB: Typical incubation at room temperature to 4°C, antibody dilutions from 1:500 to 1:5,000 depending on antigen abundance and detection system.
• IP/RIP: Incubation at 4°C with gentle rotation for 1–4 h (or overnight), followed by capture on protein A/G matrices matched to the antibody isotype.
• IF/IHC: Incubation 1–2 h at RT or overnight at 4°C; fixation and retrieval conditions depend on sample and epitope.

The validated applications, optimal dilutions, and working conditions for Ab133178 are not specified; refer to the CoA/Spec Sheet.

Safety and Handling

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS.
• General safety (typical for laboratory antibodies; defer to SDS):
  • Handle using standard laboratory PPE: lab coat, gloves (nitrile), and safety glasses.
  • Avoid inhalation of aerosols and contact with skin/eyes. Wash thoroughly after handling.
  • If formulation contains sodium azide (not specified here), avoid contact with acids (may form hydrazoic acid) and avoid disposal into copper/lead plumbing to prevent metal azide accumulation (general guidance).
• First aid (general):
  • Skin/eye contact: Rinse with water for at least 15 minutes; remove contaminated clothing; seek medical advice if irritation persists.
  • Ingestion/inhalation: Move to fresh air; rinse mouth; seek medical attention if unwell.
• Storage incompatibilities (general): Protect from microbial contamination; avoid extreme pH or high shear. Keep away from strong oxidizers and proteases.
• Product-specific handling:
  • Storage Conditions: Store at -20°C; avoid repeated freezing and thawing (per Product Data).
  • Shipping: Shipped in ice chest with ice pads (per Product Data) to maintain cold chain.

Always consult the product SDS and CoA for authoritative hazard, composition, and handling details for Ab133178.

Solvent Selection

As a protein-based reagent, this antibody is handled in aqueous buffers rather than organic solvents.

• Item-specific formulation: Not specified for this item; refer to CoA/Spec Sheet.

General buffer selection for antibody handling (literature/general):

• Preferred buffers: PBS (pH 7.2–7.4) or TBS (pH 7.4–8.0) for storage and routine assays.
• Additives: Glycerol (10–50%) can support freeze–thaw resistance when stored at −20°C; sugars (trehalose/sucrose) may stabilize lyophilized formats; BSA may reduce nonspecific adsorption. Presence/absence for this item is not specified.
• Avoid: High concentrations of primary amines (e.g., Tris) during NHS-ester conjugations; chelators (EDTA) in metal-affinity applications; azide in live-cell assays or HRP-based detection (enzyme inhibition).

Comparison (general):

• PBS: Isotonic, widely compatible, minimal buffering capacity near pH 7.4; good for most immunoassays.
• TBS: Slightly higher pH range; can reduce background in some Western blots.
• HEPES: Superior pH control near neutral for live-cell applications; avoid if amine-reactive conjugation is planned.

For Ab133178, use the exact buffer composition and any restrictions provided on the CoA/Spec Sheet when available.

Storage and Reconstitution

• Item-specific storage and shipping (from Product Data):

  • Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.
  • Shipped In: Ice chest + ice pads.

• Additional item-specific details (appearance, buffer, concentration, reconstitution volume): Not specified for this item; refer to CoA/Spec Sheet.

General handling best practices for antibodies (literature/general; not product-specific specifications):

• Upon receipt: Inspect cold-chain integrity. If the product is liquid, aliquot into low-protein-binding tubes to minimize freeze–thaw cycles. If lyophilized, spin down briefly before opening.
• Reconstitution (if lyophilized; only if indicated on CoA): Reconstitute with sterile, neutral pH buffer (e.g., PBS) to the recommended concentration; mix gently without vortexing to avoid aggregation.
• Storage: For short-term use (days to a few weeks), store at 2–8°C if permitted by formulation; for longer-term storage, −20°C or below per Product Data. Consider adding glycerol to 40–50% for −20°C storage if compatible with the formulation and application.
• Avoid: Multiple freeze–thaw cycles; high-temperature exposure; microbial contamination; and vigorous agitation.

Always follow the CoA/Spec Sheet for Ab133178 for formulation-specific reconstitution and storage instructions.

Structure and Identity

Recombinant U2AF65 Antibody (SKU: Ab133178) is a recombinant antibody directed against the U2AF65 protein target.

• Item-specific identifiers:

  • Product Name: Recombinant U2AF65 Antibody
  • Category: Antibody (research reagent)
  • Grade/Purity: Recombinant, ExactAb™, Validated (per Product Data)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable to protein antibodies; not specified for this item.

• Target identity:

  • Target name: U2AF65 (also known as U2AF2) — splicing factor that recognizes polypyrimidine tracts at 3′ splice sites (general knowledge).

• Structural features (general antibody architecture; literature/general):

  • Typical IgG architecture consists of two heavy and two light chains connected by inter-chain disulfide bonds, with variable regions conferring antigen binding and Fc region mediating effector functions.
  • Recombinant antibodies are sequence-defined proteins expressed in host cells (e.g., mammalian or other expression systems), enabling lot-to-lot consistency.

• 2D/functional description in words (general): Y-shaped glycoprotein with two antigen-binding fragments (Fabs) recognizing U2AF65 epitopes, and one Fc stem; epitope specificity and isotype for this item are not specified; refer to CoA/Spec Sheet.

Synthetic Utility

Not typically applicable. As a recombinant antibody, Ab133178 is not a building block or reagent for chemical synthesis and does not participate in conventional organic transformations.

For researchers interested in conjugation chemistry (general guidance; not product-specific):

• Antibody labeling can be performed via amine-reactive (NHS ester) or thiol-reactive (maleimide) chemistries, site-specific enzymatic tags, or click approaches (azide/alkyne). Buffer composition must be carefully controlled during conjugation (e.g., avoid Tris and azide for NHS/HRP workflows). The suitability of Ab133178 for conjugation and any functional groups exposed are not specified; consult the CoA/Spec Sheet.

Target Specificity

• Target name (from Product Data): U2AF65 Antibody (targeting U2AF65/U2AF2).
• Antigen/epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Clone ID: Not specified for this item; refer to CoA/Spec Sheet.
• Host/isotype: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity and sequence region: Not specified for this item; refer to CoA/Spec Sheet.

Note: The product is designated Recombinant and ExactAb™ (per Product Data), indicating a recombinant antibody format, but detailed specificity metrics and epitope mapping are not provided here.

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant U2AF65 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.