SP600125 - Moligand™, 10mM in DMSO , Inhibitor of mitogen-activated protein kinase 10;Inhibitor of mitogen-activated protein kinase 8;Inhibitor of mitogen-activated protein kinase 9, CAS No.129-56-6, Inhibitor of mitogen-activated protein kinase 10;Inhibitor of mitogen-activated protein kinase 8;Inhibitor of mitogen-activated protein kinase 9

CAS: 129-56-6 Cat. No.: S408302 Formula: C₁₄H₈N₂O Peso molecolare: 220.233 Numero EC: 204-955-6 PubChem CID: 8515

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

Nsc75890

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

Information

In vitro

SP600125 is originally characterized as a selective ATP-competitive inhibitor of c-Jun N-terminal kinase JNK. In Jurkat T cells, SP600125 inhibits the phosphorylation of c-Jun with IC50 of 5 μM to 10 μM. In CD4+ cells, such as Th0 cells isolated from either human cord or peripheral blood, SP600125 blocks cell activation and differentiation and inhibits the expression of inflammatory genes COX-2, IL-2, IL-10, IFN-γ, and TNF-α, with IC50 of 5 μM to 12 μM. However, later studies reveal that SP600125 also suppresses aryl hydrocarbon receptor (AhR) , Mps1 , and a panel of other serine/threonine kinases, including Aurora kinase A, FLT3, MELK, and TRKA . In a mouse beta cells MIN6, SP600125 (20 μM) induces the phosphorylation of p38 MAPK and its downstream CREB-dependent promoter activation. In HCT116 cells, SP600125 (20 μM) blocks the G2 phase to mitosis transition and induces endoreplication. This ability of SP600125 is independent of JNK inhibition, but due to its inhibition of CDK1-cyclin B activation upstream of Aurora A and Polo-like kinase 1. ^[6]

In vivo

In mice, SP600125 (15 mg/kg or 30 mg/kg) significantly inhibits lipopolysaccharide (LPS)-induced TNF-α expression and anti-CD3-induced apoptosis of CD4⁺ CD8⁺ thymocytes.
Cell Data

cell lines：MDCKII cells

Concentrations：0–5 μM

Incubation Time：72 hours

Powder Purity:≥98%

Specifications

Sinonimi

Nsc75890

Specifiche e purezza

Moligand™, 10mM in DMSO

Meccanismi biochimici e fisiologici

SP600125 (Nsc75890) is a broad-spectrum JNK inhibitor for JNK1, JNK2 and JNK3 with IC50 of 40 nM, 40 nM and 90 nM in cell-free assays, respectively; 10-fold greater selectivity against MKK4, 25-fold greater selectivity against MKK3, MKK6, PKB, and PKCα, a

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Grado

Moligand™

Tipo di azione

INHIBITOR

Meccanismo d'azione

Inhibitor of mitogen-activated protein kinase 10;Inhibitor of mitogen-activated protein kinase 8;Inhibitor of mitogen-activated protein kinase 9

Nomi e identificatori

• Isomeri SMILES: C1=CC=C2C(=C1)C3=NNC4=CC=CC(=C43)C2=O
• PubChem CID: 8515
• Peso molecolare: 220.233

Obiettivi associati (umani)

MAPK10 Tchem Mitogen-activated protein kinase 10 (2 Activities)

Discover Target: MAPK10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAPK8 Tchem Mitogen-activated protein kinase 8 (1 Activities)

Discover Target: MAPK8

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAPK9 Tchem Mitogen-activated protein kinase 9 (1 Activities)

Discover Target: MAPK9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Proprietà chimiche e fisiche

• Solubilità: Solubility (25°C) In vitro DMSO: 63 mg/mL (202.33 mM); Water: Insoluble; Ethanol: Insoluble
• Punto di fusione (°C): 283°C

Riferimenti

1. Lin Zhao, Liyuan Wang, Han Wang, Tao Xi, Sijia Liu, Yang Li, Jianping Ruan, Yongqing Huang.  (2023)  Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway.  ENVIRONMENTAL TOXICOLOGY,  38  (7): (1756-1767).  [PMID:37070943] [10.1002/tox.23805]

2. Xing Ying, Liu Yang, Qi Zhong, Liu Zhengrong, Wang Xin, Zhang Hongyi.  (2021)  LAGE3 promoted cell proliferation, migration, and invasion and inhibited cell apoptosis of hepatocellular carcinoma by facilitating the JNK and ERK signaling pathway.  CELLULAR & MOLECULAR BIOLOGY LETTERS,  26  (1): (1-16).  [PMID:34837962] [10.1186/s11658-021-00295-4]

3. Bai Yu, Du Qiang, Zhang Le, Li Ling, Wang Nana, Wu Bo, Li Ping, Li Ling.  (2021)  Silencing of ANGPTL8 Alleviates Insulin Resistance in Trophoblast Cells.  Frontiers in Endocrinology,      [PMID:34163433] [10.3389/fendo.2021.635321]

4. Zhaohui Shi, Min Xu, Xiaodong Chen, Jian Wang, Tianfeng Zhao, Dingjun Zha.  (2020)  The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal epithelial cells.  EXPERIMENTAL AND MOLECULAR PATHOLOGY,      [PMID:33285209] [10.1016/j.yexmp.2020.104591]

5. Kang An, Yuehan Zhang, Yingjiao Liu, Shengxi Yan, Zhaowei Hou, Meng Cao, Guangkuo Liu, Congcong Dong, Juncha Gao, Gaifang Liu.  (2020)  Neferine induces apoptosis by modulating the ROS‑mediated JNK pathway in esophageal squamous cell carcinoma.  ONCOLOGY REPORTS,  44  (3): (1116-1126).  [PMID:32705225] [10.3892/or.2020.7675]

6. Niu Ting, Wei Zhiying, Fu Jiao, Chen Shu, Wang Ru, Wang Yuya, Zheng Ruihe.  (2023)  Venlafaxine, an anti-depressant drug, induces apoptosis in MV3 human melanoma cells through JNK1/2-Nur77 signaling pathway.  Frontiers in Pharmacology,      [PMID:36686679] [10.3389/fphar.2022.1080412]

7. Tian Xusheng, Zhang Yukun, Li Han, Li Yunfeng, Wang Ning, Zhang Wei, Ma Boyan.  (2020)  Palmatine ameliorates high fat diet induced impaired glucose tolerance.  BIOLOGICAL RESEARCH,  53  (1): (1-12).  [PMID:32928312] [10.1186/s40659-020-00308-0]

8. Yichao Du, Junjie Bai, Tingting Ma, Ziming Wu, Pengru Wang, Xiaolin Zhong, Wenguang Fu, Shuixiang He.  (2025)  Sinomenine Ameliorates Liver Fibrosis by Blocking TGF-β/SMAD and c-JUN Signaling.  PHYTOTHERAPY RESEARCH,      [PMID:40452107] [10.1002/ptr.8502]

Domande frequenti

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 129-56-6, the molecular formula is C14H8N2O, and the molecular weight is 220.233 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.