Sumo 1 Antibody - Primary antibody, specific to Sumo 1, Rabbit IgG

Cat. No.: Ab129741

GRADE & PURITY Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Sumo 1 Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000

IHC: 1/50-1/100

Protein Function:

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.

Specifications

Product Name

Sumo 1 Antibody - Primary antibody, specific to Sumo 1, Rabbit IgG

Synonyms

DAP1 antibody | GAP modifying protein 1 antibody | GAP-modifying protein 1 antibody | GMP 1 antibody | GMP1 antibody | OFC10 antibody | PIC 1 antibody | PIC1 antibody | SENP2 antibody | Sentrin 1 antibody | Sentrin antibody | Small ubiquitin related modif

Specifications & Purity

ExactAb™, Validated, 0.6 mg/mL

Host species

Rabbit

Specificity

Sumo 1

Immunogen

A synthetic peptide derived from human Sumo 1 (AA 84-96)

Positive Control

WB: HeLa, MCF7, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast carcinoma tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.6 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - Western Blot
All lanes: Sumo 1 Antibody (Ab129741) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 12 kDa
Observed band size: 18, 82 kDa
Exposure time: 56.1 s

IHC: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Sumo 1 Antibody (Ab129741). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Sumo 1 Antibody (Ab129741)

Sumo 1 Antibody (Ab129741) - IF/ICC

IF analysis of Sumo 1 Antibody (Ab129741) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Sumo 1 Antibody (Ab129741) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

SUMO1 Tbio Small ubiquitin-related modifier 1 (0 Activities)

Discover Target: SUMO1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action