TBE Buffer (5×, RNase Free) - BioReagent, Suitable for molecular biology, RNase free, for NA electrophoresis, 5×

Cat. No.: T1518783

GRADE & PURITY Suitable for molecular biology, BioReagent, RNase free, for NA electrophoresis, 5×

Synonyms

5× Tris-Borate-EDTA Buffer (RNase-Free) | 5× TBE Electrophoresis Buffer (RNase Free) | 5× TBE Concentrate (RNase-Free) | Tris-Borate-EDTA Buffer (5×, RNase Free)

Storage

Room temperature

Shipped In

Normal

Application

Nucleic Acid Electrophoresis

Panoramica

Tris-Borate-EDTA Buffer (TBE) is a commonly used nucleic acid electrophoresis buffer. The composition of 0.5× TBE working solution is: 45 mM Tris-borate, 1 mM EDTA (pH 8.0), with moderate buffering capacity, suitable for separating small DNA fragments (<2000 bp). The 0.5–1× TBE working solution offers stronger buffering capacity and can be selected according to experimental requirements.

TBE Buffer (5×, RNase Free) is a 5-fold concentrated solution prepared with DEPC-treated water. It primarily contains 450 mM Tris-borate and 10 mM EDTA. It is mainly used for RNA electrophoresis. Before use, dilute to a 0.5× working solution with DEPC-treated water.

This reagent is for research use only. Not for use in diagnostic procedures or other purposes.

Procedure (For Reference Only)

Dilute the product to a 0.5× working solution with DEPC-treated water before use.

Notes

1. 10× TBE stock solution has poor stability and is prone to precipitation. The 5× TBE concentrated solution is recommended for more stable storage.

2. Routinely use 0.5× TBE as the working solution. For applications requiring stronger buffering capacity, 0.5–1× TBE working solution can be used.

3. Strictly avoid RNase contamination throughout the entire experimental procedure.

4. Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.

5. Use the reagent as soon as possible after opening and keep it sealed to avoid affecting experimental results.

Specifications

Sinonimi

5× Tris-Borate-EDTA Buffer (RNase-Free) | 5× TBE Electrophoresis Buffer (RNase Free) | 5× TBE Concentrate (RNase-Free) | Tris-Borate-EDTA Buffer (5×, RNase Free)

Specifiche e purezza

BioReagent, Suitable for molecular biology, RNase free, for NA electrophoresis, 5×

Stabilità e conservazione

Store at room temperature long term (12 months).

Condizioni di conservazione di stoccaggio

Room temperature

Spedito in

Normal

Grado

BioReagent, for NA electrophoresis, RNase free, Suitable for molecular biology

Domande frequenti

How should this product be stored?

Store at room temperature.

What does Suitable for molecular biology mean?

Suitable for molecular biology indicates that the product has been processed and tested for molecular biology and proteomics. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.