Thy1.1/CD90.1 Mouse mAb (AF405) - Anticorpo primario, specific to Thy1.1/CD90.1

Cat. No.: Ab212905

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

consiglio, Mouse

Isotype

Mouse IgG1

Clone Number

OX-7

Conjugation

AF405

Panoramica

Mouse anti Mouse/Rat Thy1.1/CD90.1 Antibody, Monoclonal (OX-7), could be used for Flow Cytometry.

Specifications

Product Name

Thy1.1/CD90.1 Mouse mAb (AF405) - Anticorpo primario, specific to Thy1.1/CD90.1

Sinonimi

T25 | Thy-1 | Thy1.1 | Thy1.2 | Thy-1.2 | CD7 | CD90 antigen | CD90 | CDw90 | FLJ33325 | Thy-1 antigen | Thy-1 cell surface antigen | thy-1 membrane glycoprotein | Thy-1 T-cell antigen | Thy1 | CD7 antibody | CD90 antibody | CD90 antigen antibody | CDw90

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Specie ospite

Mouse

Specificità

Thy1.1/CD90.1

Immunogeno

Rat thymocyte Thy-1 antigen

Controllo positivo

Flow: rat splenocytes

Coniugazione

AF405

Eccitazione (nm)

Violet Laser(405nm)

Emissione (nm)

422nm

Ex/Em(nm)

Ex:401nm;Em:422nm

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A/G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: Thy1.1/CD90.1 Mouse mAb (AF405) (Ab212905)

Thy1.1/CD90.1 Mouse mAb (AF405) (Ab212905) - Flow Cytometry
Flow cytometry analysis of Thy1.1/CD90.1 expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with Thy1.1/CD90.1 Mouse mAb (AF405) (Ab212905) at 0.05μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Obiettivi associati (umani)

THY1 Tbio Thy-1 membrane glycoprotein (0 Activities)

Discover Target: THY1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific tested applications, dilutions, and protocols: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature; not item-specific instructions)

• Titration: Determine optimal antibody amount per test by staining a Thy1.1+ control and a negative control, plotting stain index vs. antibody dose.
• Staining buffer: Use isotonic buffer (e.g., PBS) with protein carrier; keep samples protected from light.
• Compensation/unmixing: Include single-stain AF405 controls to set compensation for 405 nm–excited detectors or to build spectral libraries.
• Fixation: Validate AF405 signal retention with your fix/perm method (some blue fluorophores are sensitive to aldehyde fixation). When uncertain, acquire data from live-stained, unfixed controls.
• Multiplexing: Check for spillover into adjacent detectors (e.g., AmCyan/Pacific Blue channels) and adjust panel design accordingly.

These are general best practices; follow your institution’s SOPs and prioritize any instructions provided in the lot-specific datasheet.

Biological Roles

General literature context for Thy1/CD90 (not item-specific specifications)

• Protein: Thy1 (CD90) is a small, GPI‑anchored cell surface glycoprotein of the immunoglobulin superfamily.
• Allelic variants: In mice, Thy1.1 (formerly AKR allele) and Thy1.2 (C57BL/6 allele) differ by a few amino acids, enabling allele‑specific antibody recognition. This underlies common congenic tracking strategies (e.g., Thy1.1 vs Thy1.2) in immunology and transplantation studies.
• Expression patterns: Thy1 is expressed on thymocytes, subsets of mature T cells, neurons, fibroblasts, and mesenchymal stromal cells; expression is context dependent (developmental stage, tissue, activation state).
• Functions: Reported roles include cell–cell adhesion, modulation of T‑cell activation, neurite outgrowth, and regulation of fibroblast/MSC phenotype via integrin and Src‑family kinase signaling. As a GPI‑anchored protein, Thy1 partitions into membrane microdomains and can act as a co‑receptor or organizer of signaling complexes.
• Research utilities: Allele‑specific anti‑Thy1.1 antibodies permit discrimination of donor vs host cells in adoptive transfer, tracking of engineered T cells, and negative selection in mixed populations.

Caveats and controls

• Strain specificity: Ensure the experimental mice express the Thy1.1 allele; many common strains (e.g., C57BL/6) are Thy1.2. Include appropriate positive (Thy1.1+) and negative (Thy1.2+) controls.
• Shedding/cleavage: Surface levels may change with activation or enzymatic processing; validate staining on the relevant biological state.

Buffer Applications

Not typically applicable. This product is an antibody reagent supplied in a ready‑to‑use biological buffer; it is not a buffering agent and is not used to prepare buffer systems.

Practical note

• For optimal performance, use isotonic staining buffers compatible with live or fixed cells (e.g., PBS with protein carrier). Buffer composition for this item is not specified; refer to CoA/Spec Sheet.

Green Alternatives

While this product is a biologic reagent rather than an organic solvent or bulk chemical, greener laboratory practices can still be applied.

Considerations and options (general; not item-specific)

• Preservatives: If sodium azide is not compatible with downstream applications or waste streams, azide‑free formulations or spin‑column buffer exchange can reduce environmental and safety burdens. Preservative content for this item is not specified; check the CoA.
• Glycerol content: Glycerol facilitates freeze‑thaw resistance but complicates cold‑chain mass and wastewater load. If supplied with glycerol (not specified here), consider aliquoting to avoid freezing and reduce glycerol use.
• Packaging: Consolidate orders to reduce cold‑chain shipments. Reuse gel packs and insulated shippers where permitted by your institution.
• Waste minimization: Optimize staining titers to reduce reagent consumption. Plan panels to minimize redundant markers and repeat runs.
• Safer dyes: AF405 is a modern fluorophore with improved brightness and photostability relative to legacy coumarins; it generally enables lower usage per test, reducing chemical load.

Quick comparison (literature-based, general)

• AF405 vs Pacific Blue: Both are 405 nm–excited; AF405 often shows good photostability and brightness in many systems. Selection should be guided by your instrument filters and spillover into adjacent channels.
• Pre-conjugated mAb vs in‑house labeling: Purchasing validated conjugates avoids excess dye handling and organic solvent waste from labeling reactions and purifications.

Pharmaceutical Uses

Not applicable. This product is provided strictly for research use only (per Product Data) and is not intended for human or veterinary diagnostic or therapeutic applications.

Formulation context (general information)

• Preclinical assay support: Fluorophore‑conjugated antibodies like AF405‑labeled anti‑Thy1.1 are commonly used in discovery-stage research, assay development, and characterization of cell populations, but they are not pharmaceutical excipients or drug substances.
• Compliance: No pharmacopeial monographs are applicable to this research reagent. For regulated workflows, qualify the reagent internally and retain lot-specific CoA/SDS.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values (for context; not item-specific specifications)

• Antibody size: Typical intact IgG ≈150 kDa; actual mass varies with isotype and glycosylation.
• AF405 spectral properties (literature):
  • Excitation max ~401 nm; emission max ~421–423 nm.
  • Suitable for violet/near-UV laser lines (e.g., 405 nm) with blue emission detectors.
• Extinction coefficient/quantum yield (AF405, literature): ε ≈35,000–40,000 M⁻1·cm⁻1; quantum yield reported ~0.3–0.4 (values vary by source and microenvironment). These are dye-only literature ranges, not specifications for this conjugate.
• Buffer and pH: Antibodies are commonly supplied in near‑physiological buffers (e.g., PBS, pH ~7.2–7.4) with stabilizers, but exact composition for this item is not specified; refer to CoA/Spec Sheet.

Practical notes

• Photostability: AF405 is more photostable than many traditional blue fluorophores but still requires light protection (aligns with Product Data: protect from light).
• Temperature sensitivity: Protein conformation and dye performance can be compromised by freeze–thaw; Product Data specifies do not freeze.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: ExactAb™, Validated.

Interpretation (general; how to use these designations)

• ExactAb™: Denotes a product line emphasizing batch-to-batch consistency, defined specificity, and controlled conjugation processes. For conjugates, this typically implies a controlled degree of labeling and rigorous removal of unconjugated dye; the exact metrics for this item are not specified—consult the CoA.
• Validated: Indicates the antibody has undergone functional testing against its stated target. Specific validated applications (e.g., flow cytometry, IF, IHC) are not specified for this item; refer to CoA/Spec Sheet or lot-specific validation data.

What to check on receipt

• CoA/Spec Sheet should detail: concentration (mg/mL), buffer composition, preservative/stabilizer content, recommended storage, and where applicable, degree of labeling (DOL) and residual free dye.
• Lot-to-lot controls: Compare performance to historical controls using your assay (e.g., maintain fixed PMT settings for cytometry and monitor MFI).

Implications for experimental design

• If your assay is quantitative, confirm linearity across dilutions and verify spillover/compensation using AF405 controls. If multiplexing, check that spectral signatures match your instrument’s 405 nm laser/detector configuration.

Reaction and Applications

This is a labeled monoclonal antibody reagent rather than a small-molecule reactant; classical organic reaction conditions are not applicable.

General research applications (literature; not item-specific promises)

• Flow cytometry: AF405 is efficiently excited by 405 nm lasers with blue emission, enabling use as a low‑spillover fluor for panels requiring separation from FITC/PE channels. Typical usage includes staining of Thy1.1/CD90.1‑positive cells for identification or sorting.
• Fluorescence microscopy/IF: AF405 provides blue emission useful for multiplex imaging where green/red channels are occupied by other markers.
• Immunoassays: May be used in plate-based fluorescence readouts if the target epitope is accessible and the optical configuration supports AF405.

Target biology context (literature)

• Thy1 (CD90) is a GPI‑anchored glycoprotein. Thy1.1 and Thy1.2 are allelic variants; antibodies specific to Thy1.1 enable discrimination of congenic mouse strains.

Practical tips (general)

• Light management: Minimize exposure; keep on ice and protected during staining.
• Titration: Determine optimal antibody units per test by titration on positive/negative controls to maximize separation index while minimizing background.
• Controls: Include fluorescence-minus-one (FMO) and isotype controls as appropriate. Use single‑stained AF405 controls for compensation/spectral unmixing.

Reaction Conditions

Not applicable. There are no chemical reaction conditions to optimize for this product, as it is an immunoreagent rather than a small‑molecule reactant or catalyst.

Operational conditions (general handling, literature)

• Staining is typically performed at 2–8 °C or room temperature for 15–45 minutes in the dark, followed by washes. Exact conditions for this item are not specified; optimize by titration in your system.
• Avoid exposure to intense light, extreme pH (<6 or >8.5), and organic solvents that can denature the antibody or quench AF405 fluorescence.

Safety and Handling

Item-specific facts (from Product Data)

• Storage: Store at 2–8 °C; protect from light; do not freeze.
• Shipping: Shipped on wet ice; do not freeze.
• GHS/SDS: Signal word, H‑statements, pictograms, and classification are not specified for this item; refer to SDS for authoritative safety information.

General safety guidance (literature/best practice; not item-specific specifications)

• Likely hazard profile: Most research-use antibody solutions are not classified as hazardous under GHS when free of harmful preservatives; however, always consult the SDS for this specific lot/formulation.
• Preservatives: Presence/absence of sodium azide, thimerosal, glycerol, or BSA is not specified for this item. Handle as if azide may be present: avoid contact with acids and plumbing containing copper/lead where azide salts may accumulate.
• PPE: Wear lab coat, suitable gloves (e.g., nitrile), and eye protection. Work with minimal exposure to light to preserve fluorophore integrity.
• Handling: Avoid microbial contamination; use aseptic technique. Do not vortex harshly; mix gently by inversion to preserve antibody integrity.
• Incompatibilities: Avoid strong oxidizers, proteases, and extremes of pH or temperature. Do not autoclave.
• First aid (general):
  • Skin/eye contact: Rinse with water for 15 minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Ingestion/inhalation: Rinse mouth, move to fresh air; seek medical advice.

Research use only: Not for human or veterinary diagnostic or therapeutic use.

Solvent Selection

This product is an aqueous antibody reagent, not a small-molecule solute.

Item-specific facts (from Product Data)

• Storage buffer composition is not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature)

• Compatibility: Antibody conjugates are typically supplied in PBS or similar isotonic buffers. Avoid organic solvents (e.g., >10% DMSO, methanol) which can denature proteins and quench fluorophores.
• Sample prep: For cell staining, maintain isotonic conditions (e.g., PBS + 0.5–2% protein carrier such as BSA or serum, and 0.05–0.1% sodium azide for fixed-cell work if compatible with downstream assays). For live cells, avoid azide.
• Filtration: If sterile use is required, 0.22 μm filtration can remove microbes but may adsorb some antibody; pre-rinse filters with buffer containing carrier protein to minimize loss.
• Surfactants: Low levels of nonionic surfactants (e.g., 0.01% Tween‑20) can reduce nonspecific binding in some assays; validate compatibility with AF405 detection and your target biology.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage temperature: Store at 2–8 °C.
• Light sensitivity: Protect from light.
• Freezing: Do not freeze.
• Shipping: Shipped on wet ice; do not freeze.

Not specified for this item; refer to CoA/Spec Sheet

• Form/appearance (liquid vs lyophilized), buffer composition, preservative content, protein concentration, and reconstitution instructions (if lyophilized) are not specified.

General handling guidance (literature; not item-specific specifications)

• Upon receipt: Inspect for leakage or turbidity. Mix gently by inversion; avoid vigorous vortexing.
• Aliquoting: If frequent use is expected and formulation permits, prepare working aliquots to reduce cumulative light and handling exposure. Do not freeze (per Product Data).
• Storage buffer: Maintain near‑neutral pH and isotonicity. If buffer exchange is needed, use gentle desalting (spin columns) and validate performance post-exchange.
• Stability monitoring: Track performance with a standard positive control; declining signal may indicate photobleaching or denaturation.

Research use only: Not for human or veterinary diagnostic or therapeutic use.

Structure and Identity

Item-specific facts (from Product Data)

• Product name: Thy1.1/CD90.1 Mouse mAb (AF405)
• Format: Mouse monoclonal antibody, AF405-conjugated
• Catalog/SKU: Ab212905
• Grade: ExactAb™, Validated
• CAS, CID, InChIKey, SMILES: Not applicable for protein biologics; not specified for this item; refer to CoA/Spec Sheet.

General/literature information (non-spec, for context)

• Antibody class: Monoclonal antibodies are Y‑shaped immunoglobulins composed of two heavy and two light chains stabilized by disulfide bonds. Common isotypes include IgG subclasses; specific isotype for this clone is not specified for this item.
• Conjugate: AF405 (Alexa Fluor 405) is a blue-excited organic fluorophore covalently linked (typically via NHS-ester chemistry) to lysine side chains on the antibody.
• Structural features: The immunoglobulin contains Fc and Fab regions; AF405 dye molecules are distributed on accessible amine residues. Degree of labeling (DOL) is not specified for this item; refer to CoA/Spec Sheet.

2D structural description (qualitative)

• Protein biopolymer with immunoglobulin fold domains (beta-sandwich). No small-molecule structural descriptors (SMILES/InChI) are applicable to the intact conjugate.

Synthetic Utility

Not applicable. This product is a fluorophore‑conjugated monoclonal antibody and is not used as a synthetic building block or reagent in chemical synthesis.

Where to look instead

• For practical use cases relevant to this antibody, see the sections: Reaction & Applications, Biological Roles, and Application Protocols (noting that protocol details for this item are not specified and should be taken from the CoA/Spec Sheet).

Target Specificity

Item-specific facts (from Product Data)

• Target/Antigen: Thy1.1/CD90.1
• Antibody type: Mouse monoclonal antibody (mAb)
• Conjugate: AF405 (Alexa Fluor 405)

Not specified for this item; refer to CoA/Spec Sheet

• Clone/designation: Not specified
• Epitope: Not specified
• Species reactivity: Not specified
• Isotype: Not specified
• Cross‑reactivity: Not specified
• Applications tested: Not specified
• Positive controls: Not specified

Notes

• The product name indicates allele specificity for Thy1.1/CD90.1; confirm strain/allele of your biological samples to ensure appropriate reactivity.