Thy1.1/CD90.1 Mouse mAb (AF555) - Anticorpo primario, specific to Thy1.1/CD90.1

Cat. No.: Ab212907

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

consiglio, Mouse

Isotype

Mouse IgG1

Clone Number

OX-7

Conjugation

AF555

Panoramica

Mouse anti Mouse/Rat Thy1.1/CD90.1 Antibody, Monoclonal (OX-7), could be used for Flow Cytometry.

Specifications

Product Name

Thy1.1/CD90.1 Mouse mAb (AF555) - Anticorpo primario, specific to Thy1.1/CD90.1

Sinonimi

T25 | Thy-1 | Thy1.1 | Thy1.2 | Thy-1.2 | CD7 | CD90 antigen | CD90 | CDw90 | FLJ33325 | Thy-1 antigen | Thy-1 cell surface antigen | thy-1 membrane glycoprotein | Thy-1 T-cell antigen | Thy1 | CD7 antibody | CD90 antibody | CD90 antigen antibody | CDw90

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Specie ospite

Mouse

Specificità

Thy1.1/CD90.1

Immunogeno

Rat thymocyte Thy-1 antigen

Controllo positivo

Flow: rat splenocytes

Coniugazione

AF555

Eccitazione (nm)

Green Laser(532nm)

Emissione (nm)

568nm

Ex/Em(nm)

Ex:553nm;Em:568nm

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A/G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: Thy1.1/CD90.1 Mouse mAb (AF555) (Ab212907)

Thy1.1/CD90.1 Mouse mAb (AF555) (Ab212907) - Flow Cytometry
Flow cytometry analysis of Thy1.1/CD90.1 expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with Thy1.1/CD90.1 Mouse mAb (AF555) (Ab212907) at 0.05μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Obiettivi associati (umani)

THY1 Tbio Thy-1 membrane glycoprotein (0 Activities)

Discover Target: THY1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Tested applications, recommended dilutions, and positive control materials are not specified for this item; refer to the CoA/Spec Sheet.

General guidance for AF555-conjugated mouse monoclonal antibodies (non-item-specific):

• Instrument setup: Use a laser/filter set compatible with AF555 excitation (~555 nm) and emission (~565–580 nm). Verify spectral overlap in multiplex panels and apply compensation as needed.
• Titration: Perform a titration on your specific cells/tissue to determine the minimal antibody amount that yields maximal specific signal with low background.
• Controls: Include isotype controls, fluorescence-minus-one (FMO) controls, and unstained samples to establish gates and thresholds.
• Handling: Protect from light during staining and washes. Keep samples on ice or at recommended temperatures to limit capping/internalization if surface staining is required.
• Fixation/permeabilization: If fixation is necessary, validate that the chosen fixative and permeabilization conditions preserve AF555 fluorescence and antigenicity.

These steps are general best practices and not performance claims for this item.

Biological Roles

Thy1 (CD90) is a glycosylphosphatidylinositol (GPI)-anchored cell-surface glycoprotein of the immunoglobulin superfamily. The Thy1.1 (CD90.1) designation refers to an allelic isoform recognized in certain inbred rodent strains (literature, general). While this product targets Thy1.1/CD90.1, the biological notes below summarize general roles of Thy1/CD90:

• Cell adhesion and signaling: Thy1 interacts with integrins and other membrane components, modulating cell adhesion, neurite outgrowth, and T-cell activation (literature, general).
• Tissue distribution: Expressed on subsets of T lymphocytes, neurons, fibroblasts, mesenchymal stromal cells, and some endothelial cells; the exact pattern depends on species and tissue context (literature, general). Allelic variants (e.g., 1.1 vs 1.2) differ by epitope and strain distribution in rodents.
• Functional pathways: Involved in cytoskeletal regulation, focal adhesion signaling, and immunological synapse dynamics; GPI anchoring localizes Thy1 to lipid rafts, influencing receptor clustering (literature, general).
• Research utility: Antibodies to Thy1.1 enable discrimination between closely related allelic variants in rodent models, facilitating adoptive transfer and chimera studies (literature, general). This is a general statement; species/strain reactivity of this specific item is not specified.

Note: These biological insights provide context for target relevance. They are literature-based and not performance claims for this item. Users should confirm species/allele specificity and application suitability empirically for their system.

Buffer Applications

Buffer preparation is not a primary focus for this product. It is supplied as an antibody reagent for immunodetection rather than as a buffering agent.

Practical notes (general):

• Working buffers commonly include PBS or similar isotonic solutions for staining, washing, and storage. The exact formulation of this item is not specified; refer to CoA/Spec Sheet.
• Include carrier proteins or low concentrations of detergents only if compatible with your assay and downstream readout; verify that additives do not increase background fluorescence.
• Maintain neutral pH and ionic strength to preserve antibody binding and AF555 fluorescence.

No specific buffer system, pH range, or recipe is provided for this item.

Green Alternatives

A “green alternatives” discussion is not typically applicable to an antibody reagent. However, environmentally mindful practices can still apply:

• Reduce, Reuse (appropriately), Recycle:
  • Minimize single-use plastics by consolidating aliquoting sessions and using recyclable tip boxes where permitted by your lab’s EHS.
  • Plan experiments to optimize antibody usage and reduce waste.
• Safer buffers: Use phosphate-based buffers with minimal hazardous additives; avoid azide-containing waste in biological drains according to local regulations.
• Energy conservation: Store at 2–8°C (as required) and avoid unnecessary freezer space since this item must not be frozen.

If your workflow requires alternative detection chemistries (e.g., enzyme labels instead of fluorophores) for lower energy instrumentation or longer reagent life, evaluate trade-offs in sensitivity, waste profile, and instrument needs. These considerations are general and not item-specific.

Pharmaceutical Uses

This product is a research-grade antibody reagent.

• Intended use: For research use only (item-specific). Not for diagnostic procedures, clinical use, or as an active pharmaceutical ingredient or excipient.
• Pharmacopeial status: Not specified for this item; refer to CoA/Spec Sheet if needed for regulatory documentation in non-clinical settings.

In pharmaceutical research labs, fluorescently labeled antibodies like this may be used in assay development, target validation, and cell characterization. These are general research applications and do not constitute clinical or therapeutic use claims.

Physical Properties

Item-specific physical-chemical specifications are not provided for this antibody.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/Protein content: Not specified for this item; refer to CoA/Spec Sheet.
• Formulation/Buffer components: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet (typical IgG ~150 kDa; literature, general).
• pH: Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality, preservatives, stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

AF555 fluorophore optical properties (literature, general for Alexa Fluor 555-like dyes):

• Excitation maximum: ~555 nm (literature)
• Emission maximum: ~565–580 nm, commonly ~565–570 nm (literature)
• Molar absorptivity (ε): ~1.5×10^5 M⁻¹·cm⁻¹ (literature)
• Quantum yield: ~0.1–0.3 depending on environment (literature)

Solubility and stability (general):

• Soluble in aqueous physiological buffers; avoid exposure to strong light to prevent fluorophore photobleaching (general guidance).
• Temperature sensitivity: Do not freeze (item-specific); store 2–8°C protected from light (item-specific).

Note: For any definitive numerical property or formulation component, consult the item’s CoA/Spec Sheet and SDS. Values above are literature/general for the fluorophore and typical IgG and are not item-specific specifications.

Quality and Grades

• Grade/Purity: ExactAb™, Validated (item-specific)

What this means (general explanation):

• ExactAb™: Denotes a manufacturer-defined quality system for monoclonal antibodies, typically indicating consistent lot-to-lot performance, stringent identity verification (e.g., target recognition by immunoassay), and controlled conjugation chemistry for labeled formats. Specific acceptance criteria for this item are not specified; refer to CoA/Spec Sheet.
• Validated: Indicates the antibody-dye conjugate has undergone in-house performance checks against defined targets and methods. Exact validated applications and species reactivity are not specified for this item.

Quality implications for the AF555 conjugate (general):

• Controlled degree of labeling (DOL) is important for signal quality and antigen binding. The DOL for this item is not specified; consult the CoA.
• Low free dye content and preservation of functional affinity help achieve high signal-to-noise in fluorescence-based assays.
• Validation typically includes assessment of specificity, background, and photostability under recommended storage and handling.

Documentation and lot control:

• For lot-specific concentration, buffer composition, DOL, and performance data, review the Certificate of Analysis/Spec Sheet.
• If your workflow is GLP/GMP-adjacent, retain lot records and verify any method suitability (e.g., instrument laser/filter compatibility for AF555) before large studies.

Reaction and Applications

This is a labeled monoclonal antibody, not a synthetic reagent. Chemical reactions and catalytic applications are not applicable.

Relevant laboratory applications (general for AF555-conjugated antibodies):

• Fluorescence-based detection of Thy1.1/CD90.1 on cell surfaces by microscopy or flow cytometry.
• Immunofluorescence staining of fixed or live cells/tissues when compatible with AF555.
• Multiplex panels where AF555 is paired with appropriate lasers/filters and spectrally distinct fluorophores.

Note: Specific tested applications for this item are not provided. Users should perform application-specific validation and titration under their conditions. Consult the CoA/Spec Sheet for any available performance guidance.

Reaction Conditions

Not applicable. This product is not designed for chemical synthesis or catalytic reactions.

Experimental conditions relevant to use (general for AF555-conjugated antibodies):

• Temperature: Perform staining at 2–8°C or room temperature as appropriate for the assay and cell viability constraints. Avoid temperatures that promote internalization unless intended. Item-specific temperature limits during storage: 2–8°C, do not freeze.
• Light protection: Minimize light exposure before and during staining to prevent photobleaching of AF555.
• Compatibility: Use optical configurations matching AF555 excitation (~555 nm) and emission (~565–580 nm) (literature, general).

No item-specific incubation times, concentrations, or buffers are provided; users should empirically optimize under their assay conditions.

Safety and Handling

Safety details specific to this item’s GHS classification and hazard statements are not provided.

• Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.
• Research use: For research use only (item-specific). Not for diagnostic or therapeutic use.

General handling guidance for fluorescently labeled antibodies:

• PPE: Wear lab coat, disposable nitrile gloves, and safety glasses. Avoid skin/eye contact and ingestion. Handle with appropriate aseptic technique if sterility is required.
• Light sensitivity: Protect from light at all times (item-specific storage condition) to minimize AF555 photobleaching.
• Temperature: Store at 2–8°C; do not freeze (item-specific). Freezing can cause aggregation and loss of activity/fluorescence.
• Incompatibilities: Avoid strong oxidizers, proteases, and extremes of pH or ionic strength that can denature protein or degrade fluorophore (general).
• Spill/cleanup: Absorb with inert material, wash area with mild detergent; dispose according to institutional guidelines for biological reagents.
• First aid (general): If contact with skin/eyes occurs, rinse with water for at least 15 minutes; if inhaled or ingested, seek medical attention and provide SDS.
• Waste: Dispose as non-hazardous aqueous lab waste unless local regulations or buffer additives dictate otherwise. Consult your EHS.

Always consult the product SDS for authoritative hazard communication and emergency measures.

Solvent Selection

This product is an aqueous antibody reagent rather than a solvent. Traditional “solvent selection” does not apply.

Practical notes (general):

• Working medium: Typically handled in physiological aqueous buffers (e.g., PBS or HBSS) with protein stabilizers or low detergent as appropriate for the assay. The exact buffer for this item is not specified; refer to CoA/Spec Sheet.
• Avoid organic solvents and pH extremes that can denature antibodies or quench AF555 fluorescence.
• For sample preparation or washing steps, choose buffers compatible with AF555 (excitation ~555 nm) and your cytometer/microscope optical filters to minimize background autofluorescence.

Storage and Reconstitution

• Storage conditions (item-specific): Store at 2–8°C. Protect from light. Do not freeze.
• Shipping (item-specific): Shipped on wet ice. Do not freeze during transit.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied as liquid, use directly. If lyophilized (not specified), reconstitute per the CoA with sterile buffer and mix gently.
• Aliquoting: If aliquoting is necessary for workflow, do so aseptically at 2–8°C and protect from light. Avoid repeated freeze–thaw cycles (item requires no freezing).
• Stability: Lot-specific shelf life and post-opening stability are not specified; consult the CoA/Spec Sheet. Monitor for precipitation, increased background, or loss of signal as indicators of degradation.

Note: Follow institutional biosafety and waste procedures. Maintain light protection during storage and handling to preserve AF555 fluorescence. For research use only.

Structure and Identity

This product is a fluorescently labeled monoclonal antibody specific to the Thy1.1/CD90.1 antigen, supplied as a Mouse mAb conjugated to AF555.

• SKU: Ab212907
• Product name: Thy1.1/CD90.1 Mouse mAb (AF555)
• Category: Antibody (research reagent)
• Grade/Purity: ExactAb™, Validated (item-specific)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• CAS, CID, InChIKey, SMILES: Not applicable/not specified for antibody reagents.

Structural features (general for this item type):

• Antibody class: Mouse monoclonal antibody (isotype/clone not specified for this item).
• Conjugate: AF555 fluorophore covalently linked to the antibody (labeling degree not specified).
• Target: Thy1.1/CD90.1 epitope (glycosylphosphatidylinositol-anchored cell-surface glycoprotein isoform). Precise epitope/affinity not specified for this item.

2D “structure” description in words (general):

• A Y‑shaped immunoglobulin protein comprising two heavy and two light chains with Fc and Fab regions; AF555 dye molecules are typically coupled to lysine side chains on the antibody surface via amide linkages. The product includes no small-molecule structure representation (SMILES/InChI) due to its macromolecular nature.

Synthetic Utility

Not applicable. This product is a fluorescently labeled monoclonal antibody and is not used as a chemical building block or reagent for organic synthesis.

If conjugation or further derivatization is contemplated (general):

• Secondary chemistry on the antibody (e.g., additional labeling) should be avoided unless you have expertise in protein conjugation; uncontrolled modification can compromise binding affinity and AF555 performance.
• Any modification details for this item (e.g., residual reactive groups) are not specified; consult the CoA/Spec Sheet.

Target Specificity

• Antigen/Target: Thy1.1/CD90.1 (item-specific, from product name)
• Host/Type: Mouse monoclonal antibody (item-specific, from product name)
• Conjugate: AF555 fluorophore (item-specific, from product name)

Not specified for this item; refer to CoA/Spec Sheet for the following details:

• Clone designation
• Isotype and light chain type
• Species reactivity and cross-reactivity profile
• Epitope mapping/affinity (KD)
• Validated applications and positive control samples

General note: Thy1.1/CD90.1 denotes an allelic variant of CD90 commonly used to distinguish specific rodent strains (literature, general). Users should verify allele and species compatibility for their experimental system.