GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Trypsin selectively hydrolyzes peptide bonds formed by the carboxyl groups of lysine or arginine in denatured proteins, making it a crucial digestive enzyme. In addition, trypsin is widely used as an adjunctive treatment for conditions such as pyothorax, hemothorax, surgical inflammation, ulcers, and traumatic injuries, particularly in addressing localized edema, hematomas, and abscesses.
Assay Principle: Trypsin catalyzes the hydrolysis of the ester bond in TAME (Nα-benzoyl-L-arginine methyl ester), releasing a free carboxyl group. This group neutralizes sodium hydroxide in the reaction system, causing a decrease in the solution's pH. Using phenol red as an indicator, the change in absorbance at 555 nm is measured, allowing for the rapid determination of trypsin activity. Within a certain range, trypsin activity exhibits a good linear relationship with the decrease in absorbance at 555 nm.
Specifications
Specifiche e purezza
BioReagent
Grado
BioReagent
Applicazione
Enzyme activity assay
Tipo di campione
Tessuti animali
Metodo di rilevamento
Colorimetric Method
Detection instrument
Visible Spectrophotometer
Detection wavelength
555nm
Stoccaggio e spedizione
Condizioni di conservazione di stoccaggio
Store at 2-8°C,Protected from light
Spedito in
Wet ice
Stabilità e conservazione
Store at 2-8℃ for 6 months. Store in the dark.
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
