UBE2C Antibody - Anticorpo primario, specific to UBE2C, Rabbit IgG

Cat. No.: Ab133227

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human UBE2C Antibody, Polyclonal, could be used for WB, ICC, IF and so on.
Application
· WB: 0.01-2μg/mL
· ICC/IF: 5-20μg/mL
Protein Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Specifications

Product Name

UBE2C Antibody - Anticorpo primario, specific to UBE2C, Rabbit IgG

Sinonimi

Cyclin selective ubiquitin carrier protein antibody | dJ447F3.2 antibody | Mitotic specific ubiquitin conjugating enzyme antibody | UB E2C antibody | UBCH 10 antibody | UbcH10 antibody | UBE 2C antibody | Ube2c antibody | UBE2C_HUMAN antibody | Ubiquitin

Specifiche e purezza

Carrier Free, ExactAb™, Validated, High Performance, See COA

Specie ospite

Coniglio

Specificità

UBE2C

Immunogeno

Recombinant UBE2C expressed in E.coli (AA 2-179).

Controllo positivo

WB: HeLa, A431, MCF7 and RAW 264.7 cell lysates. ICC/IF: HeLa cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified + Protein A purified

Concentrazione

See COA

Product Line

biological_products

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: UBE2C Antibody (Ab133227)

UBE2C Antibody (Ab133227) - Western Blot
All lanes: UBE2C Antibody (Ab133227) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 20 kDa
Observed band size: 17, 19 kDa
Exposure time: 5.0 s

IF/ICC: UBE2C Antibody (Ab133227)

UBE2C Antibody (Ab133227) - IF/ICC
FITC staining on IF
Samples: Human HeLa Cells
Primary Ab: 20 µg/mL UBE2C Antibody (Ab133227).

IF/ICC: UBE2C Antibody (Ab133227)

UBE2C Antibody (Ab133227) - IF/ICC

IF analysis of UBE2C Antibody (Ab133227) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with UBE2C Antibody (Ab133227) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Applicazione

Applicazione

Dilution info

WB

0.01-2 µg/mL

IF/ICC

5-20 µg/mL

Obiettivi associati (umani)

UBE2C Tbio Ubiquitin-conjugating enzyme E2 C (0 Activities)

Discover Target: UBE2C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UBE2C Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.