Carrier Free, ExactAb™, Validated, High performance, 1.0 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti V5 tag Antibody, Monoclonal (K05002_3F6), could be used for WB, IP and so on.
Application:
WB: 1/3000-1/4000
IP: 1/100
V5 tag Mouse mAb (Ab133823) - Western Blot
All lanes: V5 tag Mouse mAb (Ab133823) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 50, 112 kDa
Exposure time: 1.16 s
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged CDA at C-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag antibody at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: HEK293 lysate (transfected with V5 tagged CDA at C-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane1. Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged ARF3 at N-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag protein at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: HEK-293 lysate (transfected with V5 tagged ARF3 at N-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane 1.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with untransfected HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag Mouse mAb (Ab133823) at 1.0 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: Untransfected HEK293 lysate.Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by KT25.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: Unspecific immunoprecipitation by V5 tag Mouse mAb (Ab133823) was not obsearved.
| Application | Dilution info |
|---|---|
| IP | 1/100 |
| WB | 1/3000-1/4000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708830 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
| ZJ24F0708829 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
| ZJ24F0708828 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
