GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.1.0 mg/mL
Carrier Free, ExactAb™, Validated, High performance, 1.0 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Mouse anti V5 tag Antibody, Monoclonal (K05002_3F6), could be used for WB, IP and so on. Application: WB: 1/3000-1/4000 IP: 1/100
Specifications
Product Name
V5 tag Mouse mAb - Anticorpo primario, specific to V5, Mouse IgG2a
Sinonimi
GKPIPNPLLGLDST tag antibody | Protein Rev antibody | Regulator of expression of viral proteins antibody | rev antibody | V5 epitope tag antibody | GKPIPNPLLGLDST Epitope Tag | Parainfluenza virus 5 V/P | Paramyxovirus SV5 | GKPIPNPLLGLDST epitope tag anti
Specifiche e purezza
Carrier Free, ExactAb™, Validated, High performance, 1.0 mg/mL
Specie ospite
Mouse
Specificità
V5
Immunogeno
A fusion protein containing V5.
Controllo positivo
WB: HEK293 cells transfected with T7 tag. IP: HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus; HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus and KT82 was used as IgG isotype control antibody; HEK-293 cell lysates tran
Coniugazione
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Mouse IgG2a
SDS-PAGE
150 kDa
Metodo di purificazione
Protein A purified
Concentrazione
1.0 mg/mL
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -80°C,Avoid repeated freezing and thawing
Spedito in
Dry ice packs + Cold packs
Stabilità e conservazione
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
V5 tag Mouse mAb (Ab133823) - Western Blot All lanes: V5 tag Mouse mAb (Ab133823) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 50, 112 kDa Exposure time: 1.16 s
V5 tag Mouse mAb (Ab133823) - IP Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged CDA at C-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag antibody at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: HEK293 lysate (transfected with V5 tagged CDA at C-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane1. Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged ARF3 at N-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag protein at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: HEK-293 lysate (transfected with V5 tagged ARF3 at N-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane 1.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with untransfected HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag Mouse mAb (Ab133823) at 1.0 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: Untransfected HEK293 lysate.Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by KT25.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: Unspecific immunoprecipitation by V5 tag Mouse mAb (Ab133823) was not obsearved.
Applicazione
Applicazione
Dilution info
IP
1/100
WB
1/3000-1/4000
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Recensioni
Recensioni dei clienti
Domande frequenti
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (V5 tag Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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