CD8 alpha Rat mAb (APC) - Primary antibody, specific to CD8 alpha

Cat. No.: Ab190214
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
53-6.7
Conjugation
APC
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab190214-10μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
15,90US$
25μg
Ab190214-25μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
31,90US$
100μg
Ab190214-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
99,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Specifications

Product Name
CD8 alpha Rat mAb (APC) - Primary antibody, specific to CD8 alpha
Especificaciones y pureza
ExactAb™, Validated, 0.5 mg/mL
Mecanismos bioquímicos y fisiológicos
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Especies hospedadoras
Rat
Especificidad
CD8 alpha
Inmunógeno
Mouse Spleen Cells or Thymocyte Membranes
Control positivo
Flow Cytometry: C57BL/6 mouse splenocytes.
Conjugación
APC
Excitación (nm)
Red Laser(633nm)
Emisión (nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Grado
ExactAb™, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes

CD8 alpha Rat mAb (APC) (Ab190214) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (APC) (Ab190214) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC) (Ab180313).

CD8 alpha Rat mAb (APC) (Ab190214) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (APC) (Ab190214) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación
AplicaciónDilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ25F0927051Certificate of AnalysisJul 14, 2026 Ab190214
ZJ25F0927052Certificate of AnalysisJul 14, 2026 Ab190214
ZJ25F0927053Certificate of AnalysisJul 14, 2026 Ab190214
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