Aurora A Mouse mAb - Primary antibody, specific to Human AURKA

Cat. No.: Ab184689
DISPONIBLE À COMMANDE
QUALITÉ & PURETÉ Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
ELISA, Flow Cytometry, IF/ICC, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2b
Clone Number
EF01/3C12
Conjugation
Unconjugated
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Taille
USA
Allemagne (EU)*
Prix
Qté
10μl
Ab184689-10μl
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
99,90$US
50μl
Ab184689-50μl
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
259,90$US
100μl
Ab184689-100μl
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
419,90$US
1ml
Ab184689-1ml
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
3 399,90$US
Enter a quantity for the sizes you want to add.
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Pourquoi cette qualité

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

Mouse anti Human AURKA Antibody, clone EF01/3C12, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Spécifications

Product Name
Aurora A Mouse mAb - Primary antibody, specific to Human AURKA
Synonymes
AIK antibody | ARK-1 antibody | ARK1 antibody | AURA antibody | Aurka antibody | Aurora 2 antibody | Aurora A antibody | Aurora kinase A antibody | Aurora-related kinase 1 antibody | Aurora/IPL1 like kinase antibody | AURORA/IPL1-like kinase antibody | Au
Spécifications et pureté
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
Mécanismes biochimiques et physiologiques
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mit
Espèce hôte
Mouse
Spécificité
Human AURKA
Immunogène
Recombinant protein corresponding to Human AURKA
Contrôle positif
WB: MCF-7 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Type de chaîne lumineuse
kappa
SDS-PAGE
150.0 KDa
Méthode de purification
Protein A purified
Concentration
1.0 mg/mL
Product Line
Produits biologiques
Stockage et expédition
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Aurora A Mouse mAb (Ab184689) - Western Blot
All lanes: Aurora A Mouse Antibody (Ab184689) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 42 kDa
Exposure time: 37.3 s 

Aurora A Mouse mAb (Ab184689) - Flow Cytometry ​
Flow Cytometry analysis of HeLa cells stained with Aurora A  Mouse mAb (Ab184689) (Red) at 0.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue). ​

Aurora A Mouse mAb (Ab184689) - IF/ICC
IF analysis of Aurora A (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Aurora A Mouse mAb (Ab184689) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application
Application
Dilution info
WB

1/1000 - 1/2000

IF/ICC

1/200

Flow Cytometry

1/1000 - 1/10000

Documentation

📋 Fiche de données de sécurité (FDS)

Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.

<<<<<<< HEAD Télécharger la SDS → ======= Télécharger la SDS → >>>>>>> release248-p3

✅ Certificat d'analyse (COA)

Données qualité spécifiques au lot. Saisissez votre numéro de lot pour récupérer le COA exact.

Rechercher le COA →

📊 Fiche technique

Résumé de référence rapide des spécifications et applications du produit.

Voir la fiche technique →

🔬 Fiche de spécifications

Attributs de qualité complets et critères d'acceptation pour cette qualité.

Voir la fiche de spécifications →

Données avancées

Cibles associées (humaines)
AURKA Tchem Aurora kinase A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calculateurs de solution
Avis

Avis des clients

Foire aux questions

What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Aurora A Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

Besoin d'aide pour choisir la qualité ?

Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.

Voir le guide des qualités Carrier Free → Voir le guide des qualités Validated → Voir le guide des qualités ExactAb™ → Voir le guide des qualités Azide Free → Voir le guide des qualités High Performance → Voir le guide des qualités PBS Only →

Produits apparentés

Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - Isotype Control Antibody, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - Secondary antibody, IgG
BALB/c Mouse IgG - Isotype Control Antibody, IgG
Rabbit Anti-Mouse IgG antibody - Secondary antibody
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody
Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody
Rabbit Anti-Mouse IgG (HRP) - Secondary antibody
Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - Secondary antibody, Goat IgG
Mouse IgG (Biotin) - Isotype Control Antibody, Mouse IgG
Mouse IgG (PE) - Isotype Control Antibody, Mouse IgG
Mouse IgG (APC) - Isotype Control Antibody, Mouse IgG
Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG
Mouse IgG2a-Fc - Isotype Control Antibody, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - Isotype Control Antibody, IgG
Mouse IgG (PerCP) - Isotype Control Antibody, IgG
Mouse IgG (AF405) - Isotype Control Antibody, IgG
IgG de souris (AF647) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
IgG de souris (AF700) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF700) - Secondary antibody, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - Isotype Control Antibody
Mouse IgG (PE-Cy7) - Isotype Control Antibody
Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Mouse IgG2a-kappa, anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - Isotype Control Antibody
Mouse IgG2a-Fc (FITC) - Isotype Control Antibody
Mouse IgG (AF555) - Isotype Control Antibody
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - Secondary antibody, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - Primary antibody, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - Secondary antibody
Goat Anti-Mouse IgG H&L (PE-Cy7) - Secondary antibody
Mouse IgG (PE-Cy5.5) - Isotype Control Antibody
Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody
Mouse IgG2a-kappa (AF555), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - Isotype Control Antibody, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - Secondary antibody
Goat Anti-Mouse IgG H&L (Oregon Green 488) - Secondary antibody
Goat Anti-Mouse IgG H&L (Cy3) - Secondary antibody
Mouse IgG2a-kappa (APC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG (PE-AF647) - Isotype Control Antibody

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