Mouse IgG (AF555) - Isotype Control Antibody

Cat. No.: Ab190246

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Isotype

Mouse IgG

Conjugation

AF555

Specifications

Product Name

Mouse IgG (AF555) - Isotype Control Antibody

Synonymes

Mouse IgG | AF555-conjugated Mouse Polyclonal antibody | AF555 Mouse IgG - Isotype Control

Spécifications et pureté

ExactAb™, Validated, 0.5 mg/mL

Espèce hôte

Mouse

Conjugation

AF555

Excitation(nm)

Green Laser(532nm)

Émission (nm)

568nm

Ex/Em(nm)

Ex:553nm;Em:568nm

Grade

ExactAb™, Validated

Méthode de purification

Affinity chromatography

Concentration

0.5 mg/mL

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Mouse IgG (AF555) (Ab190246)

Mouse IgG (AF555) (Ab190246) - Flow Cytometry
Flow cytometry analysis of CD11b expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD11b Antibody (AF555) (Ab213133) at 0.1μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Mouse IgG (AF555) (Ab190246)

Mouse IgG (AF555) (Ab190246) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Mouse mAb (AF555) (Ab210002) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.; Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mouse IgG (AF555)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.