Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG

Cat. No.: Ab180307

QUALITÉ & PURETÉ ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Isotype

Mouse IgG

Conjugation

FITC

Vue d’ensemble

Application:
Flow: ≤1μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Spécifications

Product Name

Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG

Synonymes

Mouse IgG | FITC-conjugated Mouse Polyclonal antibody | FITC Mouse IgG - Isotype Control

Spécifications et pureté

ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 0.5 mg/mL

Espèce hôte

Mouse

Conjugation

FITC

Excitation(nm)

Blue Laser(488nm)

Émission (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grade

Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated

Isotype

Mouse IgG

Méthode de purification

Affinity chromatography

Concentration

0.5 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Mouse IgG Isotype Control Antibody (FITC) (Ab180307)

Mouse IgG Isotype Control Antibody (FITC) (Ab180307) - Flow Cytometry
Flow cytometry analysis of CD13 expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD13 Mouse mAb (FITC) (Ab181138) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (FITC) (Ab180307).

Flow Cytometry: Mouse IgG Isotype Control Antibody (FITC) (Ab180307)

Mouse IgG Isotype Control Antibody (FITC) (Ab180307) - Flow Cytometry
Flow cytometry analysis of CD13 expression by Human peripheral blood granulocytes (red) compared to an isotype control (blue). Cells were stained with CD13 Mouse mAb (FITC) (Ab181138) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

≤1.0μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. 

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mouse IgG (FITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.