Caveolin-1 Mouse mAb - Primary antibody, specific to Caveolin-1, Mouse IgG1

Cat. No.: Ab156204

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human, Rat

Isotype

Mouse IgG1

Clone Number

CD01/7F2

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human Caveolin-1 Antibody, clone CD01/7F2, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on.

Spécifications

Product Name

Caveolin-1 Mouse mAb - Primary antibody, specific to Caveolin-1, Mouse IgG1

Synonymes

BSCL3 antibody | CAV antibody | CAV1 antibody | CAV1_HUMAN antibody | caveolae protein, 22 kD antibody | caveolin 1 alpha isoform antibody | caveolin 1 beta isoform antibody | Caveolin 1 caveolae protein 22kDa antibody | Caveolin-1 antibody | Caveolin1 an

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),0.5 mg/mL

Mécanismes biochimiques et physiologiques

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-

Espèce hôte

Mouse

Spécificité

Caveolin-1

Immunogène

Recombinant protein corresponding to Human Caveolin-1

Contrôle positif

WB: HUVEC and C6 whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150.0 KDa

Méthode de purification

Protein A purified

Concentration

0.5 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: Caveolin-1 Mouse mAb (Ab156204)

Caveolin-1 Mouse mAb (Ab156204) - Western Blot
All lanes: Caveolin-1 Mouse Antibody (Ab156204) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 21 kDa
Exposure time: 30.0 s

Flow Cytometry: Caveolin-1 Mouse mAb (Ab156204)

Caveolin-1 Mouse mAb (Ab156204) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Caveolin-1 Mouse mAb (Ab156204) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

ICC/IF: Caveolin-1 Mouse mAb (Ab156204)

Caveolin-1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caveolin-1 Mouse mAb (Ab156204) at 2.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application

Dilution info

IF/ICC

1/200

Flow

1/200 - 1/25,000

WB

1/1,000; Predicted molecular weight: 20.5 KDa

Cibles associées (humaines)

CAV1 Tbio Caveolin-1 (0 Activities)

Discover Target: CAV1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Caveolin-1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.