CD20 Mouse mAb (Biotin) - Primary antibody, specific to CD20

Cat. No.: Ab191692

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human, Monkey

Isotype

Mouse IgG2b

Clone Number

2H7

Conjugation

Biotin

Vue d’ensemble

Mouse anti Human/Monkey CD20 Antibody, Monoclonal (2H7), could be used for Flow, IHC, IP, in vivo B cell depletion in hCD20 Tg mice and so on.

Specifications

Product Name

CD20 Mouse mAb (Biotin) - Primary antibody, specific to CD20

Synonymes

FMC7 | B1 | B-lymphocyte antigen CD20 | B-lymphocyte cell-surface antigen B1 | B-lymphocyte surface antigen B1 | Biotin  Anti-Human CD20 (2H7) | Biotin Anti-Human CD20 Antibody[2H7] | CD20 Monoclonal Antibody (2H7), Biotin | Biotin Mouse Anti-Human CD20 |

Spécifications et pureté

ExactAb™, Validated, 0.5 mg/mL

Mécanismes biochimiques et physiologiques

This protein may be involved in the regulation of B-cell activation and proliferation.

Espèce hôte

Mouse

Spécificité

CD20

Immunogène

Human tonsillar B cells

Contrôle positif

Flow: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Type de chaîne lumineuse

kappa

Méthode de purification

Protein A purified

Concentration

0.5 mg/mL

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD20 Mouse mAb (Biotin) (Ab191692)

CD20 Mouse mAb (Biotin) (Ab191692) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD20 Mouse mAb (Biotin) (Ab191692) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: CD20 Mouse mAb (Biotin) (Ab191692)

CD20 Mouse mAb (Biotin) (Ab191692) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD20 Mouse mAb (Biotin) (Ab191692) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Cibles associées (humaines)

MS4A1 Tclin B-lymphocyte antigen CD20 (0 Activities)

Discover Target: MS4A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action