CD25 Mouse mAb - Primary antibody, specific to Human CD25

Cat. No.: Ab169225

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

CD01/3A10

Conjugation

Unconjugated

Overview

Mouse anti Human CD25 Antibody, clone CD01/3A10, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

CD25 Mouse mAb - Primary antibody, specific to Human CD25

Synonyms

Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor subunit alpha antibody | IL-2-RA antibody | IL-2R subunit alpha antibody | IL2 RA antibody | IL2 R

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Biochemical and Physiological Mechanisms

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Host species

Mouse

Specificity

Human CD25

Immunogen

Recombinant protein corresponding to Human CD25

Positive Control

Flow Cytometry: Jurkat cells, Human peripheral blood lymphocytes.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Light Chain Type

kappa

SDS-PAGE

150.0 KDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488).

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150).

Flow Cytometry: CD25 Mouse mAb (Ab169225)

CD25 Mouse mAb (Ab169225) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with CD25  Mouse mAb (Ab169225) (Red) at 1. 25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557)was used as an isotype control (Blue).

Application

• Application: Dilution info
• Flow Cytometry:

  1/200 - 1/3000

Associated Targets(Human)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)

Discover Target: IL2RA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)