CD28 Mouse mAb - Primary antibody, specific to CD28, Mouse IgG1

Cat. No.: Ab094407
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
Activation, Flow Cytometry, Functional Assay, Stimulation
Host species
Mouse
Reactive species
Mouse
Isotype
Mouse IgG1
Clone Number
D665
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab094407-10μg
5
$67.90
50μg
Ab094407-50μg
2
$199.90
100μg
Ab094407-100μg
1
$299.90
1mg
Ab094407-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Mouse CD28 Antibody, Monoclonal (D665), could be used for Flow, in vivo T cell stimulation/activation, in vitro T cell stimulation/activation and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo T cell stimulation/activation: Use at an assay dependent concentration.
in vitro T cell stimulation/activation: Use at an assay dependent concentration.
Protein Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Specifications

Product Name
CD28 Mouse mAb - Primary antibody, specific to CD28, Mouse IgG1
Synonyms
CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell
Specifications & Purity
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Host species
Mouse
Specificity
CD28
Immunogen
A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images

CD28 Mouse mAb (Ab094407) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD28 with CD28 Mouse mAb (Ab094407) conjugated with biotin (right panel) at 5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

CD28 Mouse mAb (Ab094407) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD28 (red) with CD28 Mouse mAb (Ab094407) conjugated with biotin at 5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

Use at an assay dependent concentration.

in vitro T cell stimulation/activation

Use at an assay dependent concentration

in vitro T cell stimulation/activation

Use at an assay dependent concentration.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD28 Tbio T-cell-specific surface glycoprotein CD28 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0910537Certificate of AnalysisApr 07, 2026 Ab094407
ZJ24F0910538Certificate of AnalysisApr 07, 2026 Ab094407
ZJ24F0910539Certificate of AnalysisApr 07, 2026 Ab094407
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View PBS Only grade guide →

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