CD28 Mouse mAb (PE) - Primary antibody, specific to CD28, Mouse IgG1

Cat. No.: Ab185380
DISPONIBLE À COMMANDE
QUALITÉ & PURETÉ ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Ex:565nm ? Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:575nm ? Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
CD28.2
Conjugation
PE
★
Taille
USA
Allemagne (EU)*
Prix
Qté
10T
Ab185380-10T
En stock ≥10
—
59,90$US
25T
Ab185380-25T
En stock ≥10
—
119,90$US
100T
Ab185380-100T
2 En stock
—
339,90$US
Enter a quantity for the sizes you want to add.
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Pourquoi cette qualité

ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test Em:575nm,Ex:565nm,ExactAb™,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at 2-8°C,Protected from light,Do not freeze Expédition Wet ice,Do not freeze. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Spécifications

Product Name
CD28 Mouse mAb (PE) - Primary antibody, specific to CD28, Mouse IgG1
Synonymes
CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell
Spécifications et pureté
ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test
Espèce hôte
Mouse
Spécificité
CD28
Immunogène
Human CD28 transfected cell line
Contrôle positif
Flow: Jurkat
Conjugation
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Émission (nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grade
Em:575nm, Ex:565nm, ExactAb™, Validated
Isotype
Mouse IgG1
Type de chaîne lumineuse
kappa
Méthode de purification
Protein G purified
Concentration
5 μL/test
Product Line
Produits biologiques
Stockage et expédition
Conditions de stockage de stockage
Store at 2-8°C,Protected from light,Do not freeze
Expédié en
Wet ice,Do not freeze
Stabilité et stockage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD28 Mouse mAb (PE) (Ab185380) - Flow Cytometry
Flow cytometry analysis of CD28 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD28 Mouse mAb (PE) (Ab185380) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Application
Application
Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Fiche de données de sécurité (FDS)

Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.

<<<<<<< HEAD Télécharger la SDS → ======= Télécharger la SDS → >>>>>>> release248-p3

✅ Certificat d'analyse (COA)

Données qualité spécifiques au lot. Saisissez votre numéro de lot pour récupérer le COA exact.

Rechercher le COA →

📊 Fiche technique

Résumé de référence rapide des spécifications et applications du produit.

Voir la fiche technique →

🔬 Fiche de spécifications

Attributs de qualité complets et critères d'acceptation pour cette qualité.

Voir la fiche de spécifications →

Données avancées

Cibles associées (humaines)
CD28 Tbio T-cell-specific surface glycoprotein CD28 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mécanismes d'action
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateArticle
ZJ24F0606923ZJ24F0606923Certificate of AnalysisOct 08, 2026 Ab185380
ZJ24F0606924ZJ24F0606924Certificate of AnalysisOct 08, 2026 Ab185380
ZJ24F0606925ZJ24F0606925Certificate of AnalysisOct 08, 2026 Ab185380
ZJ24R0500629ZJ24R0500629Certificate of AnalysisMay 31, 2024 Ab185380
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Foire aux questions

What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD28 Mouse mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

Besoin d'aide pour choisir la qualité ?

Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.

Voir le guide des qualités Validated → Voir le guide des qualités ExactAb™ → Voir le guide des qualités Ex:565nm → Voir le guide des qualités Em:575nm →

Produits apparentés

Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - Isotype Control Antibody, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - Secondary antibody, IgG
BALB/c Mouse IgG - Isotype Control Antibody, IgG
Rabbit Anti-Mouse IgG antibody - Secondary antibody
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody
Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody
CD28 Mouse mAb - Primary antibody, specific to CD28, Mouse IgG1
FR104 (anti-CD28) - Primary antibody, specific to CD28, >95%, Human IgG2
Rabbit Anti-Mouse IgG (HRP) - Secondary antibody
Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - Secondary antibody, Goat IgG
Mouse IgG (Biotin) - Isotype Control Antibody, Mouse IgG
Mouse IgG (PE) - Isotype Control Antibody, Mouse IgG
Mouse IgG (APC) - Isotype Control Antibody, Mouse IgG
Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG
Mouse IgG2a-Fc - Isotype Control Antibody, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG
CD28 Mouse mAb (APC) - Primary antibody, Mouse IgG1
CD28 Mouse mAb (Biotin) - Primary antibody, specific to CD28, Mouse IgG1
CD28 Mouse mAb (FITC) - Primary antibody, specific to CD28, Mouse IgG1
Mouse IgG2b-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG1(D265A)
Theralizumab (anti-CD28) - Primary antibody, specific to CD28, Human IgG4
Mouse IgG (AF488) - Isotype Control Antibody, IgG
Mouse IgG (PerCP) - Isotype Control Antibody, IgG
Mouse IgG (AF405) - Isotype Control Antibody, IgG
IgG de souris (AF647) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
IgG de souris (AF700) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF700) - Secondary antibody, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - Isotype Control Antibody
Mouse IgG (PE-Cy7) - Isotype Control Antibody
Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Mouse IgG2a-kappa, anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - Isotype Control Antibody
Mouse IgG2a-Fc (FITC) - Isotype Control Antibody
Mouse IgG (AF555) - Isotype Control Antibody
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - Secondary antibody, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - Primary antibody, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - Secondary antibody
Goat Anti-Mouse IgG H&L (PE-Cy7) - Secondary antibody
Mouse IgG (PE-Cy5.5) - Isotype Control Antibody
Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody
Mouse IgG2a-kappa (AF555), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - Isotype Control Antibody, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - Secondary antibody
Goat Anti-Mouse IgG H&L (Oregon Green 488) - Secondary antibody
Goat Anti-Mouse IgG H&L (Cy3) - Secondary antibody
Mouse IgG2a-kappa (APC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG (PE-AF647) - Isotype Control Antibody

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