CD32 Mouse mAb - Primary antibody, specific to CD32, Mouse IgG2b

Cat. No.: Ab094575

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

IV.3

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human CD32 Antibody, Monoclonal (IV.3), could be used for Flow and so on.

Application

Flow: 1.0 μg/100w cells

Protein Function

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.

Spécifications

Product Name

CD32 Mouse mAb - Primary antibody, specific to CD32, Mouse IgG2b

Synonymes

CD32 antigen | CD32B | CD32Fc fragment of IgG, low affinity II, receptor for (CD32) | CDw32 | Fc fragment of IgG, low affinity IIb, receptor (CD32) | Fc fragment of IgG, low affinity IIb, receptor for (CD32) | Fc gamma RII | Fc gamma RIIb | FCG2 | Fc-gamm

Spécifications et pureté

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Espèce hôte

Mouse

Spécificité

CD32

Immunogène

The FcR of K562 cells

Contrôle positif

Flow: Human peripheral blood lymphocytes.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG2b

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD32 Mouse mAb (Ab094575)

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD32 with CD32 Mouse mAb (Ab094575) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD32 Mouse mAb (Ab094575)

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD32 (red) with CD32 Mouse mAb (Ab094575) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD32 Mouse mAb (Ab094575)

CD32 Mouse mAb (Ab094575) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD32 (red) with CD32 Mouse mAb (Ab094575) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

1μg/100w cells

Cibles associées (humaines)

FCGR2A Tbio Low affinity immunoglobulin gamma Fc region receptor II-a (0 Activities)

Discover Target: FCGR2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD32 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.