CD33 Mouse mAb (Biotin) - Primary antibody, specific to CD33, Mouse IgG1

Cat. No.: Ab181121

GRADE & PURITY Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

HIM3-4

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD33 Mouse mAb (Biotin) - Primary antibody, specific to CD33, Mouse IgG1

Synonyms

CD33 antigen (gp67) | CD33 antigen | CD33 molecule | CD33 | FLJ00391 | gp67 | myeloid cell surface antigen CD33 | p67 | sialic acid binding Ig-like lectin 3 | Sialic acid-binding Ig-like lectin 3 | Siglec3 | Siglec-3 | SIGLEC3gp67 | CD 33 antibody | CD33

Specifications & Purity

Validated, 0.5 mg/mL

Host species

Mouse

Specificity

CD33

Immunogen

NFMY-9s human cell line.

Positive Control

Flow: Human peripheral blood monocytes

Conjugation

Biotin

Grade

Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD33 Mouse mAb (Biotin) (Ab181121)

CD33 Mouse mAb (Biotin) (Ab181121) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD33 Mouse mAb (Biotin) (Ab181121) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD33 Mouse mAb (Biotin) (Ab181121)

CD33 Mouse mAb (Biotin) (Ab181121) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD33 Mouse mAb (Biotin) (Ab181121) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD33 Tclin Myeloid cell surface antigen CD33 (0 Activities)

Discover Target: CD33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action