CD33 Mouse mAb (Biotin) - Primary antibody, specific to CD33, Mouse IgG1

Cat. No.: Ab181121

QUALITÉ & PURETÉ Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

HIM3-4

Conjugation

Biotin

Vue d’ensemble

Application:
Flow: ≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Spécifications

Product Name

CD33 Mouse mAb (Biotin) - Primary antibody, specific to CD33, Mouse IgG1

Synonymes

CD33 antigen (gp67) | CD33 antigen | CD33 molecule | CD33 | FLJ00391 | gp67 | myeloid cell surface antigen CD33 | p67 | sialic acid binding Ig-like lectin 3 | Sialic acid-binding Ig-like lectin 3 | Siglec3 | Siglec-3 | SIGLEC3gp67 | CD 33 antibody | CD33

Spécifications et pureté

Validated, 0.5 mg/mL

Espèce hôte

Mouse

Spécificité

CD33

Immunogène

NFMY-9s human cell line.

Contrôle positif

Flow: Human peripheral blood monocytes

Conjugation

Biotin

Grade

Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

Méthode de purification

Protein A purified

Concentration

0.5 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD33 Mouse mAb (Biotin) (Ab181121)

CD33 Mouse mAb (Biotin) (Ab181121) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD33 Mouse mAb (Biotin) (Ab181121) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD33 Mouse mAb (Biotin) (Ab181121)

CD33 Mouse mAb (Biotin) (Ab181121) - Flow Cytometry
Flow cytometry analysis of CD33 expression by Human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD33 Mouse mAb (Biotin) (Ab181121) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Cibles associées (humaines)

CD33 Tclin Myeloid cell surface antigen CD33 (0 Activities)

Discover Target: CD33

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action