CD4 Mouse mAb (Biotin) - Primary antibody, specific to CD4, Mouse IgG2b

Cat. No.: Ab180130

QUALITÉ & PURETÉ Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

OKT4

Conjugation

Biotin

Vue d’ensemble

Application:
Flow: ≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Spécifications

Product Name

CD4 Mouse mAb (Biotin) - Primary antibody, specific to CD4, Mouse IgG2b

Synonymes

CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti

Spécifications et pureté

Validated, 0.5 mg/mL

Espèce hôte

Mouse

Spécificité

CD4

Immunogène

Human peripheral T cells.

Contrôle positif

Flow: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

Validated

Isotype

Mouse IgG2b

Méthode de purification

Protein G purified

Concentration

0.5 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD4 Mouse mAb (Biotin) (Ab180130)

CD4 Mouse mAb (Biotin) (Ab180130) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (Biotin) (Ab180130) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD4 Mouse mAb (Biotin) (Ab180130)

CD4 Mouse mAb (Biotin) (Ab180130) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (Biotin) (Ab180130) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Cibles associées (humaines)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.