CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1

Cat. No.: Ab094962
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. See COA
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
B6H12
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab094962-10μg
10
$53.90
50μg
Ab094962-50μg
4
$157.90
100μg
Ab094962-100μg
2
$243.90
1mg
Ab094962-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human CD47 Antibody, Monoclonal (B6H12), could be used for Flow and so on.

Application:

Flow: 1.0 μg/100w cells

Protein Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Specifications

Product Name
CD47 Mouse mAb - Primary antibody, specific to CD47, Mouse IgG1
Synonyms
antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein |
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA
Host species
Mouse
Specificity
CD47
Immunogen
Purified human placental RGD-binding proteins
Positive Control
Flow: Human peripheral blood lymphocytes.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, PBS Only, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.
Images

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 with CD47 Mouse mAb (Ab094962) conjugated with biotin (right panel) at 2.0 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab170221) - Isotype Control (left panel) and detected with SA-PE (rp156150). 

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD47 (red) with CD47 Mouse mAb (Ab094962) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of MCF-7 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of A549 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD47 Mouse mAb (Ab094962) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD47 (red) with CD47 Mouse mAb (Ab094962) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

1μg/100w cells

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505965Certificate of AnalysisFeb 25, 2025 Ab094962
ZJ24F0505966Certificate of AnalysisFeb 25, 2025 Ab094962
ZJ24F0505964Certificate of AnalysisMay 22, 2024 Ab094962
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View PBS Only grade guide →

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