CD74 Mouse mAb (Biotin) - Primary antibody, specific to CD74, Mouse IgG1

Cat. No.: Ab185159

QUALITÉ & PURETÉ ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

LN2

Conjugation

Biotin

Vue d’ensemble

Flow: ≤1.0 μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Spécifications

Product Name

CD74 Mouse mAb (Biotin) - Primary antibody, specific to CD74, Mouse IgG1

Synonymes

CD74 Monoclonal Antibody (LN2), Biotin | Biotin Anti-Human CD74 Antibody[LN2] | Biotin Mouse Anti-Human CD74 | Biotin anti-human CD74 Antibody | Protein 41 antibody | p35 antibody | p33 antibody | MHC HLA-DR gamma chain antibody | MHC HLA DR gamma chain a

Spécifications et pureté

ExactAb™, Validated, 0.5 mg/mL

Espèce hôte

Mouse

Spécificité

CD74

Immunogène

SU-DHL-4 cells.

Contrôle positif

Flow: Human peripheral blood lymphocytes, Raji

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Méthode de purification

Protein G purified

Concentration

0.5 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Cibles associées (humaines)

CD74 Tchem HLA class II histocompatibility antigen gamma chain (0 Activities)

Discover Target: CD74

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD74 Tchem HLA-DR antigens-associated invariant chain (25 Activities)

Discover Target: CD74

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD74 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.