Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
TSA, or Tyramide Signal Amplification, is a signal amplification technique based on HRP (Horseradish Peroxidase)-catalyzed reactions. It is commonly used in IHC (Immunohistochemistry), ICC (Immunocytochemistry), ISH (In Situ Hybridization), FISH (Fluorescence In Situ Hybridization), and ...
The washing step in IHC directly affects two types of outcomes. One is whether unbound or weakly bound detection reagents can be sufficiently removed. The other is whether specifically formed binding complexes can be stably retained.
Antigen retrieval in IHC determines the extent to which epitopes masked after fixation can be re-exposed, and it directly affects localization quality, signal intensity, and tissue integrity.
Immunohistochemistry Protocol
Immunohistochemistry (IHC) is a technique that uses the principle of specific binding between antigens and antibodies to locate, characterize and quantify antigens (peptides and proteins) within tissue cells by chemically reacting the marker antibody's color developer (fluorescein, enzyme, metal ...
IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?
What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F?
How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
Automated Immunohistochemical Staining Protocol for Anti-PD-L1
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...
Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning.
Antibodies are powerful research tools used in a variety of laboratory techniques. Here, we will provide a brief overview of the most popular laboratory techniques, focusing on how they use antibodies.
Techniques often explored alongside immunological experiments.
