How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
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How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
·Reviewed·Published ·Updated ·8 min read
How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
First of all, customers need to estimate the amount of antibody to be used according to the antibody specification & pre-test results, different samples, due to the different expression of antigen, the antibody dilution ratio will be different. For example: for a certain antibody IHC experiment, the pre-test confirms that according to the dilution ratio of 1:50, under normal circumstances, a slice needs to add 100μl of diluted antibody, that is, a slice needs 2μl of antibody, 20μl can do about 10 slices.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "How many slices can I do with 20μI when I have IHC lab-applicable antibodies?" Aladdin Knowledge Base, updated Aug 23, 2024. https://www.aladdinsci.com/us_en/faqs/how-many-slices-can-i-do-with-lab-applicable-antibodies-en.html
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