MHC class I/H-2Kb Mouse mAb - Primary antibody, specific to MHC class I/H-2Kb, Mouse IgG2a

Cat. No.: Ab115278

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Flow Cytometry, Functional Assay

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

AF6-88.5.5.3

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for in vivo administration, Flow and so on.

Application:

in vivo administration: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.

Specifications

Product Name

MHC class I/H-2Kb Mouse mAb - Primary antibody, specific to MHC class I/H-2Kb, Mouse IgG2a

Synonymes

K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espèce hôte

Mouse

Spécificité

MHC class I/H-2Kb

Immunogène

C57BL/6 mouse splenocytes

Contrôle positif

Mouse splenocyte suspensions.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG2a

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: MHC class I/H-2Kb Mouse mAb (Ab115278)

MHC class I/H-2Kb Mouse mAb (Ab115278) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC class I/H-2Kb with MHC class I/H-2Kb Mouse mAb (Ab115278) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: MHC class I/H-2Kb Mouse mAb (Ab115278)

MHC class I/H-2Kb Mouse mAb (Ab115278) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC class I/H-2Kb (red) with MHC class I/H-2Kb Mouse mAb (Ab115278) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo administration:

  Use at an assay dependent concentration;

Cibles associées (humaines)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MHC class I/H-2Kb Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.