PCNA Mouse mAb - Primary antibody, specific to PCNA, IgG2a

Cat. No.: Ab181179
DISPONIBLE À COMMANDE
QUALITÉ & PURETÉ Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 0.5 mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2a
Clone Number
C12/17A3
Conjugation
Unconjugated
★
Taille
USA
Allemagne (EU)*
Prix
Qté
10μl
Ab181179-10μl
En stock ≥10
—
45,90$US
50μl
Ab181179-50μl
1 En stock
—
199,90$US
100μl
Ab181179-100μl
1 En stock
—
299,90$US
1ml
Ab181179-1ml
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
1 499,90$US
Enter a quantity for the sizes you want to add.
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Pourquoi cette qualité

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

Application
WB: 1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa
Flow: 0.032 μg/mL - 1.0 μg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Spécifications

Product Name
PCNA Mouse mAb - Primary antibody, specific to PCNA, IgG2a
Synonymes
Cyclin | PCNA
Spécifications et pureté
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL
Espèce hôte
Mouse
Spécificité
PCNA
Immunogène
Full length recombinant human PCNA
Contrôle positif
WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
IgG2a
Type de chaîne lumineuse
kappa
SDS-PAGE
185 kDa
Méthode de purification
Protein A purified
Concentration
0.5 mg/mL
Product Line
Produits biologiques
Stockage et expédition
Forme
Liquid
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

PCNA Mouse mAb (Ab181179) - Western Blot
All lanes: PCNA Antibody (Ab181179) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 31.0 kDa
Exposure time: 54.2 s 

PCNA Mouse mAb (Ab181179) - Flow Cytometry
Overlay histogram showing HeLa cells stained with PCNA Mouse mAb (Ab181179) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Application
Application
Dilution info
WB

1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.

Flow Cytometry

0.032 μg/mL - 1.0 μg/mL

Documentation

📋 Fiche de données de sécurité (FDS)

Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.

<<<<<<< HEAD Télécharger la SDS → ======= Télécharger la SDS → >>>>>>> release248-p3

✅ Certificat d'analyse (COA)

Données qualité spécifiques au lot. Saisissez votre numéro de lot pour récupérer le COA exact.

Rechercher le COA →

📊 Fiche technique

Résumé de référence rapide des spécifications et applications du produit.

Voir la fiche technique →

🔬 Fiche de spécifications

Attributs de qualité complets et critères d'acceptation pour cette qualité.

Voir la fiche de spécifications →

Données avancées

Cibles associées (humaines)
PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mécanismes d'action
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateArticle
ZJ24F0102680ZJ24F0102680Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102681ZJ24F0102681Certificate of AnalysisDec 16, 2025 Ab181179
ZJ24F0102682ZJ24F0102682Certificate of AnalysisDec 16, 2025 Ab181179
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Besoin d'aide pour choisir la qualité ?

Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.

Voir le guide des qualités Carrier Free → Voir le guide des qualités Validated → Voir le guide des qualités ExactAb™ → Voir le guide des qualités Azide Free → Voir le guide des qualités High Performance → Voir le guide des qualités PBS Only →

Produits apparentés

Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - Isotype Control Antibody, generated in Rat, >95%, IgG
PCNA Mouse mAb - Primary antibody, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1
Rabbit Anti-Mouse IgG H&L (HRP) - Secondary antibody, IgG
Recombinant PCNA Antibody - Primary antibody, specific to PCNA, Rabbit IgG
BALB/c Mouse IgG - Isotype Control Antibody, IgG
Rabbit Anti-Mouse IgG antibody - Secondary antibody
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody
Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody
Rabbit Anti-Mouse IgG (HRP) - Secondary antibody
Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - Secondary antibody, Goat IgG
Mouse IgG (Biotin) - Isotype Control Antibody, Mouse IgG
Mouse IgG (PE) - Isotype Control Antibody, Mouse IgG
Mouse IgG (APC) - Isotype Control Antibody, Mouse IgG
Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG
Mouse IgG2a-Fc - Isotype Control Antibody, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG
Mouse IgG2b-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - Isotype Control Antibody, IgG
Mouse IgG (PerCP) - Isotype Control Antibody, IgG
Mouse IgG (AF405) - Isotype Control Antibody, IgG
IgG de souris (AF647) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
IgG de souris (AF700) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF700) - Secondary antibody, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - Isotype Control Antibody
Mouse IgG (PE-Cy7) - Isotype Control Antibody
Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Mouse IgG2a-kappa, anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - Isotype Control Antibody
Mouse IgG2a-Fc (FITC) - Isotype Control Antibody
Mouse IgG (AF555) - Isotype Control Antibody
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - Secondary antibody, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - Primary antibody, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - Isotype Control Antibody, specific to A29L
Recombinant PCNA Antibody - Primary antibody, specific to PCNA
Mouse IgG3-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - Secondary antibody
Goat Anti-Mouse IgG H&L (PE-Cy7) - Secondary antibody
Mouse IgG (PE-Cy5.5) - Isotype Control Antibody
Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody
Mouse IgG2a-kappa (AF555), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - Isotype Control Antibody, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - Secondary antibody
Goat Anti-Mouse IgG H&L (Oregon Green 488) - Secondary antibody
Goat Anti-Mouse IgG H&L (Cy3) - Secondary antibody
Mouse IgG2a-kappa (APC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG (PE-AF647) - Isotype Control Antibody

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