PP2A-alpha Antibody - Primary antibody, specific to PP2A-alpha, Rabbit IgG

Cat. No.: Ab122718

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Validated, 2.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human PP2A-alpha Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/100-1/500
Protein Function:
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.

Spécifications

Product Name

PP2A-alpha Antibody - Primary antibody, specific to PP2A-alpha, Rabbit IgG

Synonymes

PP2A alpha antibody | PP2A-alpha antibody | PP2AA_HUMAN antibody | PP2Aalpha antibody | PP2Ac antibody | PP2CA antibody | PP2Calpha antibody | PPP2CA antibody | Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody | Protein phospha

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 2.0 mg/mL

Espèce hôte

Rabbit

Spécificité

PP2A-alpha

Immunogène

A KLH conjugated synthetic peptide from the C-terminal region of human PP2A alpha (AA 274-304).

Contrôle positif

WB: A431, K562, NIH/3T3 and C6 cell lysates. IHC: Human cancer tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Saturated ammonium sulfate precipitation

Concentration

2.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PP2A-alpha Antibody (Ab122718)

PP2A-alpha Antibody (Ab122718) - Western Blot
All lanes: PP2A-alpha Antibody (Ab122718) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 48.1 s

IHC: PP2A-alpha Antibody (Ab122718)

PP2A-alpha Antibody (Ab122718) - IHC
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of PP2A-alpha Antibody (Ab122718) for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

IF/ICC: PP2A-alpha Antibody (Ab122718)

PP2A-alpha Antibody (Ab122718) - IF/ICC

IF analysis of PP2A-alpha Antibody (Ab122718) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PP2A-alpha Antibody (Ab122718) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IHC

1/100-1/500

WB

1/1000

IF/ICC

1/200

Cibles associées (humaines)

PPP2CA Tchem Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (0 Activities)

Discover Target: PPP2CA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PP2A-alpha Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.