Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b

Cat. No.: Ab188096
DISPONIBLE À COMMANDE
QUALITÉ & PURETÉ Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2b
Clone Number
FG01/2C1
Conjugation
Unconjugated
★
Taille
USA
Allemagne (EU)*
Prix
Qté
10μl
Ab188096-10μl
En stock ≥10
—
79,90$US
50μl
Ab188096-50μl
7 En stock
—
259,90$US
100μl
Ab188096-100μl
2 En stock
—
419,90$US
1ml
Ab188096-1ml
Fabrication sur commande aux États-Unis · 2–4 semaines ·
—
2 799,90$US
Enter a quantity for the sizes you want to add.
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Pourquoi cette qualité

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

WB: 1/1000 – 1/100,000

Flow: 1/200 - 1/2,000

ICC/IF: 1/200

Spécifications

Product Name
Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Synonymes
DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
Spécifications et pureté
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Espèce hôte
Mouse
Spécificité
Smad3
Immunogène
Recombinant full length protein corresponding to Human Smad3
Contrôle positif
WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells ICC/IF: U-2 OS cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
Mouse IgG2b
Type de chaîne lumineuse
kappa
SDS-PAGE
150.0 KDa
Méthode de purification
Protein A purified
Concentration
1.0 mg/mL
Product Line
Produits biologiques
Stockage et expédition
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 49 kDa
Exposure time: 0.9 s 

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 29.6 s 

Samd3 Mouse mAb (Ab188096) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Samd3 Mouse mAb (Ab188096) - ICC/IF
IF analysis of Samd3 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Samd3 Mouse mAb (Ab188096) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application
Application
Dilution info
Flow Cytometry

1/200-1/2000

IF/ICC

1/200

WB

1/1000 – 1/100,000

Documentation

📋 Fiche de données de sécurité (FDS)

Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.

<<<<<<< HEAD Télécharger la SDS → ======= Télécharger la SDS → >>>>>>> release248-p3

✅ Certificat d'analyse (COA)

Données qualité spécifiques au lot. Saisissez votre numéro de lot pour récupérer le COA exact.

Rechercher le COA →

📊 Fiche technique

Résumé de référence rapide des spécifications et applications du produit.

Voir la fiche technique →

🔬 Fiche de spécifications

Attributs de qualité complets et critères d'acceptation pour cette qualité.

Voir la fiche de spécifications →

Données avancées

Cibles associées (humaines)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mécanismes d'action
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateArticle
ZJ24F0607491ZJ24F0607491Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607492ZJ24F0607492Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607493ZJ24F0607493Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24R0600677ZJ24R0600677Certificate of AnalysisJun 14, 2024 Ab188096
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Besoin d'aide pour choisir la qualité ?

Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.

Voir le guide des qualités Carrier Free → Voir le guide des qualités Validated → Voir le guide des qualités ExactAb™ → Voir le guide des qualités Azide Free → Voir le guide des qualités High Performance → Voir le guide des qualités PBS Only →

Produits apparentés

Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - Isotype Control Antibody, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - Secondary antibody, IgG
BALB/c Mouse IgG - Isotype Control Antibody, IgG
Rabbit Anti-Mouse IgG antibody - Secondary antibody
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody
Recombinant SMAD3 Antibody - Primary antibody, specific to SMAD3, Rabbit IgG
Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody
Rabbit Anti-Mouse IgG (HRP) - Secondary antibody
Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody
Goat Anti-Mouse IgG H&L (HRP) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - Secondary antibody, Goat IgG
Mouse IgG (Biotin) - Isotype Control Antibody, Mouse IgG
Mouse IgG (PE) - Isotype Control Antibody, Mouse IgG
Mouse IgG (APC) - Isotype Control Antibody, Mouse IgG
Mouse IgG (FITC) - Isotype Control Antibody, Mouse IgG
Mouse IgG2a-Fc - Isotype Control Antibody, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - Secondary antibody, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - Secondary antibody, Goat IgG
Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG1
Smad3 Mouse mAb - Primary antibody, specific to Smad3, Mouse IgG2b
Mouse IgG2b-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG2a(D265A)
Mouse IgG1(D265A)-kappa, anti-HEL - Isotype Control Antibody, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - Isotype Control Antibody, IgG
Mouse IgG (PerCP) - Isotype Control Antibody, IgG
Mouse IgG (AF405) - Isotype Control Antibody, IgG
IgG de souris (AF647) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF555) - Secondary antibody, specific to Mouse IgG, Goat IgG
IgG de souris (AF700) - Isotype Control Antibody, IgG
Goat Anti-Mouse IgG H&L (AF700) - Secondary antibody, specific to Mouse IgG, Goat IgG
Mouse IgG (PE-Cy5) - Isotype Control Antibody
Mouse IgG (PE-Cy7) - Isotype Control Antibody
Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Mouse IgG2a-kappa, anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - Isotype Control Antibody
Mouse IgG2a-Fc (FITC) - Isotype Control Antibody
Mouse IgG (AF555) - Isotype Control Antibody
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - Secondary antibody, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - Primary antibody, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - Secondary antibody
Goat Anti-Mouse IgG H&L (PE-Cy7) - Secondary antibody
Mouse IgG (PE-Cy5.5) - Isotype Control Antibody
Goat Anti-Mouse IgG H&L (AF750) - Secondary antibody
Mouse IgG2a-kappa (AF555), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - Isotype Control Antibody, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - Secondary antibody
Goat Anti-Mouse IgG H&L (Oregon Green 488) - Secondary antibody
Goat Anti-Mouse IgG H&L (Cy3) - Secondary antibody
Mouse IgG2a-kappa (APC), anti-A29L - Isotype Control Antibody, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - Isotype Control Antibody, specific to HEL
Mouse IgG (PE-AF647) - Isotype Control Antibody

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