STAT5a Antibody - Primary antibody, specific to STAT5a, Rabbit IgG

Cat. No.: Ab129464

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Pig

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human STAT5a Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.01-2 µg/mL

IHC: 5-20 µg/mL

IF/ICC: 2.5  µg/mL

Protein Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Specifications

Product Name

STAT5a Antibody - Primary antibody, specific to STAT5a, Rabbit IgG

Synonymes

Mammary gland factor antibody | MGF antibody | Signal transducer and activator of transcription 5A antibody | STA5A_HUMAN antibody | STAT 5 antibody | STAT 5A antibody | STAT5 antibody | STAT5A antibody | MGF | signal transducer and activator of transcrip

Spécifications et pureté

Carrier Free, ExactAb™, Validated, High Performance, See COA

Espèce hôte

Rabbit

Spécificité

STAT5a

Immunogène

Recombinant STAT5a expressed in E.coli (AA 673-773)

Contrôle positif

WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Antigen affinity purified and Protein A purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: STAT5a Antibody (Ab129464)

STAT5a Antibody (Ab129464) - Western Blot
All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 24.2 s

IHC: STAT5a Antibody (Ab129464)

STAT5a Antibody (Ab129464) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: STAT5a Antibody (Ab129464)

STAT5a Antibody (Ab129464) - IF/ICC 

IF analysis of STAT5a Antibody (Ab129464) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with STAT5a Antibody (Ab129464) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

2.5 µg/mL

Cibles associées (humaines)

STAT5A Tchem Signal transducer and activator of transcription 5A (0 Activities)

Discover Target: STAT5A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

STAT5A Tchem Signal transducer and activator of transcription 5A (227 Activities)

Discover Target: STAT5A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

STAT5A Tchem STAT5A/STAT5B (8 Activities)

Discover Target: STAT5A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (STAT5a Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.