COX IV Mouse mAb - 一次抗体, specific to COX4I1, Mouse IgG1

Cat. No.: Ab097414

GRADE & PURITY ExactAb™, Validated, 2.0 mg/mL

Application

Flow Cytometry, IF/ICC, IHC, IP, WB

Host species

マウス

Reactive species

ハムスター, マウス, ヤギ, 人間, 助言, 猿

Isotype

Mouse IgG1

Clone Number

4D11-B3-E8

Conjugation

Unconjugated

概要

Mouse anti Human COX IV Antibody, Monoclonal antibody(4D11-B3-E8), could be used for WB, IHC, ICC, IF, Flow, IP and so on.

Application:

WB: 1/1000 

IHC: 1/50-1/200

ICC/IF: 1/100-1/200 

Flow: 1/100 

IP: 1/20 

Protein Function

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Specifications

Product Name

COX IV Mouse mAb - 一次抗体, specific to COX4I1, Mouse IgG1

同義語

AL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi

仕様と純度

ExactAb™, Validated, 2.0 mg/mL

宿主種

マウス

特異性

COX4I1

免疫原

A synthetic peptide derived from human COX IV (AA 1-169).

ポジティブコントロール

WB: Jurkat, NIH/3T3, HeLa, C6, K562, MCF7, THP-1, Raji; IHC: Human colorectal carcinoma; ICC/IF: HeLa cells; IP: HeLa.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Mouse IgG1

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

2.0 mg/mL

Product Line

biological_products

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5.0 s

ICC/IF: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - ICC/IF
Immunocytochemistry analysis of COX IV (green) in HeLa cells using COX IV Mouse mAb (Ab097414).

IHC: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV Mouse mAb (Ab097414). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IP: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IP
Immunoprecipitation analysis of COX IV (4D11) in HeLa cell lysates using COX IV Mouse mAb (Ab097414)

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

IP

1/20

WB

1/1000

IHC

1/50-1/200

IF/ICC

1/100-1/200

Flow Cytometry

1/100

関連ターゲット（人間）

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)

Discover Target: COX4I1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (COX IV Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.