Loading control antibodies are used to detect stable reference proteins and provide internal references for target protein quantification, sample-to-sample comparison, evaluation of subcellular fractions, and multiplex detection. Their applicability depends on reference protein stability, sample ...
Topic
Subcellular Fractionation
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Subcellular fractionation is a biological experimental technique that allows researchers to separate and purify different components within cells, such as the nucleus, mitochondria, endoplasmic reticulum, etc., and then further study their structure and function. Centrifugation is one of the ...
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Techniques often explored alongside immunological experiments.