Loading control antibodies are used to detect stable reference proteins and provide internal references for target protein quantification, sample-to-sample comparison, evaluation of subcellular fractions, and multiplex detection. Their applicability depends on reference protein stability, sample ...
Topic
Loading control
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
2 resources · protocols, FAQs & articles
Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
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Techniques often explored alongside immunological experiments.