3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
This SOP is intended for the standardized application of SYTO 9 and PI combined live/dead staining in microbial viability evaluation. It is suitable for suspended bacterial samples such as Escherichia coli and Staphylococcus aureus, and can also be extended to membrane integrity stratification ...
This procedure is intended for the standardized use of the two far-red nuclear dyes DRAQ5 and DRAQ7. It is applicable to live-cell nuclear labeling, dead-cell identification, nuclear counterstaining of fixed samples, and immunofluorescence-associated detection on fluorescence microscopy, ...
This protocol describes the establishment of angiogenesis models stimulated by fibroblast growth factor 2 (FGF2, also termed bFGF). The models are intended for assessing pro-angiogenic activity, screening inhibitors, dissecting FGFR downstream signaling, and interrogating matrix-dependent ...
Giemsa staining is a composite Romanowsky-type method based primarily on azure dyes and eosin. It is widely used for cytomorphologic evaluation of cells in peripheral blood and bone marrow smears and for microscopic detection of pathogens such as Plasmodium spp.
The electrophoretic mobility shift assay (EMSA), also known as the gel shift assay, is a classic in vitro technique used to detect DNA–protein or RNA–protein interactions under non-denaturing polyacrylamide gel electrophoresis (native PAGE) conditions.
PAS Staining (Periodic Acid–Schiff, PAS) Standard Operating Procedure (SOP)
Masson’s trichrome staining is used to differentiate and visualize the distribution and relative proportion of tissue components in paraffin-embedded tissue sections.
RNA pull-down is performed by incubating biotin-labeled bait RNA with a protein lysate to form RNA–protein complexes. The complexes are then enriched using the biotin–streptavidin system on streptavidin magnetic beads or streptavidin agarose. After multi-step washing to remove nonspecific ...
DNA pull-down is an in vitro method for detecting and enriching DNA–protein interactions.
