Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Connecting multiple peptide fragments to form an extended peptide chain is referred to as ligation. Click chemistry offers a convenient method for creating peptide-peptide linkages. By introducing an alkyne group to one peptide fragment and an N-terminal azide moiety to another, a triazole ...
As a prevalent click reaction, strain-promoted alkyne-azide cycloaddition (SPAAC) operates without the need for metal catalysts, reducing agents, or enduring ligands. Instead, it harnesses the enthalpy liberated from the inherent ring strain of cyclooctyne to facilitate the formation of stable ...
The azide-alkyne Huisgen cycloaddition is a 1,3-dipolar cycloaddition that involves the reaction between an azide and a terminal or internal alkyne, leading to the formation of a 1,2,3-triazole. Rolf Huisgen was a trailblazer in understanding the wide applicability of this organic reaction.
Click Chemistry serves as a potent technique for investigating the intracellular distribution of small molecules. Unveiling the precise cellular destinations of these compounds provides profound insights into their mechanisms of action. This methodology has been extensively employed in various ...
Techniques often explored alongside immunological experiments.
