Learn how to select, prepare, and use DBCO PEG Maleimide (DBCO-PEG-Mal) for bioorthogonal conjugation and thiol-reactive PEGylation in research applications.
Topic
SPAAC
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Guidelines for safe handling, storage, and click-chemistry applications of 1-Azido-2-(2-(2-methoxyethoxy)ethoxy)ethane (A487268) from Aladdin Scientific.
As a prevalent click reaction, strain-promoted alkyne-azide cycloaddition (SPAAC) operates without the need for metal catalysts, reducing agents, or enduring ligands. Instead, it harnesses the enthalpy liberated from the inherent ring strain of cyclooctyne to facilitate the formation of stable ...
Related topics
Techniques often explored alongside immunological experiments.